不同程度间歇低氧对3T3-L1脂肪细胞炎性细胞因子和脂肪因子的影响

【摘要】目的 测定不同程度间歇低氧（IH）处理的3T3-L1脂肪细胞中炎性细胞因子和脂肪因子水平的变化。 方法 建立阻塞性睡眠呼吸暂停（OSA）模式间歇低氧/再氧合（IH/ROX）细胞模型，将分化成熟的3T3-L1脂肪细胞分为5组，即3个不同程度IH组（5%O₂，7.5%O₂，10%O₂，编号为IH-1，IH-2，IH-3）、持续低氧（10%O₂，CH）组和正常氧对照（21%O₂，IN）组。采用ELISA法测定脂肪细胞上清液中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、瘦素和脂联素的水平，Western blot测定脂肪细胞中低氧诱导因子（HIF）-1α、葡萄糖转运蛋白（Glut）-1水平，Real-time-PCR测定脂肪细胞中HIF-1α,lut-1、TNF-α、IL-6、瘦素、脂联素的mRNA表达水平。 结果 IH组和CH组TNF-α、IL-6和瘦素蛋白及其mRNA水平均高于IN组，IH-1组TNF-α、IL-6和瘦素蛋白及瘦素mRNA水平高于IH-2、IH-3组（均P＜0.05）；IH组和CH组脂联素蛋白及其mRNA水平均低于IN组，IH-1组低于IH-2、IH-3组（均P＜0.05）。 结论 OSA模式IH与脂肪细胞炎性细胞因子和脂肪因子的表达和释放有关，IH可能是脂肪细胞炎症反应的基础，参与OSA患者胰岛素抵抗的发生。

Effect of Different Degrees of Intermittent Hypoxia on inflammatory Cytokines and Adipokines in 3T3-L1 Adipocytes

[Abstract]Objective To study the effect of different degrees of intermittent hypoxia (IH) on inflammatory cytokinesand adipokines in3T3-L1adipocytes.Methods An intermittent hypoxia/reoxygenation (IH/ROX) from obstructive sleepapnea (OSA) adipocyte model was established.3T3-L1adipocytes were divided into five groups, three IH groups (5% O2,7.5% O2 and10% O2, referred to as IH-1, IH-2and IH-3), sustained hypoxia group (10% O2, CH) and the normal oxygencontrol group (21%O2, IN). ELISA method was used to detect values of tumor necrosis factor alpha (TNF-α), interleukin-6(IL-6), leptin and adiponectin in cell supernatant. Western blot analysis was used to detect levels of hypoxia-inducible factor-1α(HIF-1α) and glucose transporter-1(Glut-1). RT-PCR assay was used to analyze HIF-1α, Glut-1, TNF-α, IL-6,leptin and adiponectin mRNA expression levels in adipocytes.Results The expression levels of TNF-α, IL-6and leptinwere significantly higher in IH and CH groups than those of IN group (P＜0.05). The expression levels of TNF-α, IL-6andleptin protein and leptin mRNA were significantly higher in IH-1group than those of IH-2and IH-3groups (P＜0.05). Theadiponectin and its mRNA levels were significantly lower in IH and CH groups than those of IN group (P＜0.05). The adiponectin level was significantly lower in IH-1group than that of IH-2and IH-3groups (P＜0.05).ConclusionThese resultsdemonstrate that IH is related to the extensive changes in the expression and release of inflammation-related adipokines incultured adipocytes. IH from OSA may underlie the development of the inflammatory response in adipocytes, which is involved in insulin resistance in patients with OSA.