大豆异黄酮抗血小板聚集作用的实验研究

目的 ]研究大豆异黄酮对血小板聚集性的影响 .方法 ]普通饲料中添加 2 0 g/kg大豆异黄酮混合物 ,饲喂GK/Jcl糖尿病大鼠 2 0周 ,测定其血小板聚集率 .利用ODS柱层析和高效液相色谱技术分离大豆异黄酮 ,体外观察大豆异黄酮单体对人血小板聚集率的影响 .结果 ]长期食用大豆异黄酮的GK/Jcl糖尿病大鼠血小板聚集率显著降低 .在人体外血小板聚集实验中 ,大豆异黄酮均抑制腺嘌呤核苷二磷酸钠、胶原蛋白和肾上腺素诱导的血小板聚集 ,大豆异黄酮类型中金雀异黄素的最强 ,其 5 0 %抑制浓度值分别为 0 38,0 30 ,0 2 3mmol/L .结论 ]大豆异黄酮具有血小板聚集抑制效应 ,在大豆抗血小板聚集中起着重要作用 .

Inhibitory effect of soybean isoflavones on platelet aggregation

OBJECTIVE?To study the influence of soybean isoflavones on platelet aggregation.?METHODS?GK/Jcl diabetic rats were fed with containing 20?g/kg of soybean isoflavones for 20 weeks, then the rates of platelet aggregation were investigated. Soybean isoflavones were isolated by ODS column chromatography and high performance liquid chromatography, and investigated for the inhibitory effects on human platelet aggregation in vitro .?RESULTS?Among the GK/Jcl rats fed soybean isoflavones, the rates of platelet aggregation were noticeably decreased. In human platelet aggregation assay in vitro , isoflavones inhibited platelet aggregation induced by ADP, collagen and epinephrine, while genistein showed the strongest inhibitory activity with the IC 50 value of 0.38, 0.30 and 0.23?mmol/L, respectively.?CONCLUSION?Soybean isoflavones has the function of decreasing platelet aggregation rates.