中药制剂预防放射性直肠炎37例临床观察

目的 研究野生型p53基因转染卵巢癌SKOV－3细胞对放疗敏感性的影响。方法 用脂质体介导的转染技术，将人野生型p53 cDNA的真核表达重组质粒分别导入受不同剂量放射照射的SKOV－3培养细胞中，观察p53不同状态下对肿瘤细胞放疗敏感性的差异。结果 SKOV－3、SKOV－3－vect及SKOV－3－p53经2Gy照射后，其集落形成数分别下降了18．6％、22．9％及44．5％；经4Gy照射后，其集落形成数分别下降了63．6％、64．9％及88．9％。转染p53基因后，肿瘤细胞S期和G2／M期的比例下降，G1／G0期的比例增加，p53基因的转染使卵巢癌细胞发生G1期阻滞。结论 外源性野生型p53基因的转染，增加了卵巢癌SKOV－3细胞对放疗的敏感性。

Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells

Objective To study the effect of wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells. Methods Recombinant eukaryotic expression vector pcDNA3 containing full-length human wild-type p53 cDNA was introduced by lipofectamine-mediated gene transfection into cultured SKOV-3 cells which had been irradiated with 2 and 4 Gy X-rays,respectively.The radiosensitivities of the tumor cells with different p53 status were studied. Results The number of colonies in the SKOV-3,SKOV-3-vect,and SKOV-3-p53 groups decreased by 18.6%,22.9% and 44.5%,respectively with 2 Gy irradiation,and decreased by 63.6%,64.9% and 88.9%,respectively with 4 Gy irradiation.After introduction of p53 cDNA,the cell number in S phase and the ratio of G-2/M phase of tumor cells decreased and the ratio of G-1/G-0 phase increased.The introduction of p53 gene into cells led to cell cycle arrest in G-1 phase. Conclusion Exogenous introduction of wild-type p53 cDNA into SKOV-3 ovarian cancer cells can increase their radiosensitivity.;