| 荧光原位杂交技术检测乳腺癌HER-2基因扩增实验方法的比较 |
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| 引用本文： | 鲁萍,张谷. 荧光原位杂交技术检测乳腺癌HER-2基因扩增实验方法的比较[J]. 中华肿瘤防治杂志, 2011, 18(1): 26-28 |
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| 作者姓名： | 鲁萍 张谷 |
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| 作者单位： | 1. 湖州中心医院病理科,浙江,湖州,313000
2. 浙江省肿瘤医院病理科,浙江,杭州,310022 |
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| 摘 要： | 目的:探讨荧光原位杂交(FISH)技术检测乳腺癌HER-2基因的最佳实验方法.方法:应用FISH技术检测55例乳腺癌组织标本的HER-2基因,分别采用水浴法和直接消化法,甲酰胺法和共变性法,检测HER-2基因表达成功率,对比实验方法的最佳结果.结果:两种消化法中,水浴法和直接法杂交成功率分别为86.21%(25/29)和88.46%(23/26),差异无统计学意义(P>0.05),杂信号比分别为17.24%(5/29)和46.15%(12/26),差异有统计学意义(P<0.05).两种变性法中甲酰胺法和共变性法杂交成功率分别为73.08%(19/26)和93.10%(27/29),差异有统计学意义(P<0.05).结论:FISH检测乳腺癌HER-2基因,以水浴法和共变性法杂交成功率最高.
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| 关 键 词： | 乳腺肿瘤 基因表达 原位杂交,荧光 |
Experimental comparison of fluorescence in situ hybridization in breast cancer HER-2 gene detection |
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| LU Ping,ZHANG Gu. Experimental comparison of fluorescence in situ hybridization in breast cancer HER-2 gene detection[J]. Chinese Journal of Cancer Prevention and Treatment, 2011, 18(1): 26-28 |
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| Authors: | LU Ping ZHANG Gu |
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| Affiliation: | LU Ping1,ZHANG Gu21.Department of Pathology,Central Hospital of Huzhou,Huzhou 313000,P.R.China 2.Department of Pathology,Zhejiang Cancer Hospital,Hangzhou 310022,P.R.China |
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| Abstract: | OBJECTIVE:To investigate the optimal methods of fluorescence in situ hybrdization in HER-2 gene detection.METHOD: Totally 55 samples of breast cancer were gathered by bath and direct digestion,dimethylformamide and denaturation law,were compared by detecting the success expression rate of HER-2 gene.RESULTS:In two digest methods,the success rates of hybridization were 86.21%(25/29)and 88.46%(23/26),respectively and with no difference(P>0.05).But hybridization signal ratio were 17.24%(5/29) and 46.15%(12/26)... |
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| Keywords: | breast neoplasms gene expression in stiu hybidization fluorescence |
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