槲皮素对人胃癌细胞侵袭和MMP-2表达的影响

目的:观察槲皮素(Quercetin,Que)对人胃癌细胞侵袭的影响,并探讨其可能机制.方法:采用不同浓度的槲皮素处理胃癌BGC-823细胞后,以软琼脂集落培养试验检测癌细胞锚着不依赖性增殖,以Boyden小室模型方法检测癌细胞侵袭能力,采用荧光实时定量PCR检测癌细胞基质金属蛋白酶-2(matrix metallopeptidase-2,MMP-2)基因mRNA水平,以Western blot方法检测癌细胞MMP-2基因蛋白水平变化.结果:不同浓度的胃癌BGC-823细胞经槲皮素处理后,恶性增殖和侵袭能力均明显下降,且呈剂量依赖性(P<0.005,P<0.005).槲皮素处理组MMP-2基因mRNA和蛋白水平均明显下调,且呈时间和浓度依赖性,即随着作用时间的延长和槲皮素作用浓度的增加,MMP-2的mRNA和蛋白水平逐渐下降,差异均有统计学意义(P<0.001,P<0.001).结论:槲皮素可明显抑制胃癌BGC-823细胞侵袭能力,其机制可能与下调MMP-2基因表达有关.

Quercetin reduces cell invasion and down-regulates matrix metallopeptidase 2 expression in human gastric cancer cell line BGC-823

AIM:To investigate the effect of quercetin on the migration of human gastric cancer BGC-823 cells and to explore possible mechanisms involved.METHODS:After BGC-823 cells were treated with different doses of quercetin,the anchorageindependent growth of cells was evaluated by colony formation assay,cell invasion ability was determined by Boyden chamber assay,and the mRNA and protein expression of matrix metallopeptidase-2 (MMP-2) was detected by realtime RT-PCR and Western blot,respectively.RESULTS:Quercetin reduced the invasion ability and anchorage-independent growth of BGC-823 cells in a dose-dependent manner (P < 0.005,P < 0.005).Treatment with quercetin down-regulated MMP-2 mRNA and protein expression in a time-and dose-dependent manner (P < 0.001,P < 0.001).CONCLUSION:Quercetin inhibits the invasion of human gastric carcinoma cell line BGC-823 possibly by down-regulating MMP-2 expression.