In vitro antioxidant and protective effects of corn peptides on ethanol-induced damage in HepG2 cells |
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Authors: | Xingxing She Fei Wang Jun Ma Xi Chen |
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Affiliation: | 1. Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, People's Republic of China;2. Beijing Engineering Research Center of Protein &3. Functional Peptides, China National Research Institute of Food &4. Fermentation Industries, Beijing 100015, People's Republic of China |
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Abstract: | To assess the effect of corn peptides (CPs) as an antioxidant on ethanol (EtOH)-induced oxidative stress in HepG2 cells, the cells were preincubated with CPs for 4?h before treated with EtOH. Methyl thiazolyl tetrazolium and flow cytometry assay were performed to detect the viability of CP-treated HepG2 cells and control cells. The activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by corresponding kits, respectively. The results showed that treatment with 300?mmol/L EtOH for 24?h resulted in a death of around 50% of the HepG2 cells. Pretreatments of appropriate concentrations of CPs (more than 5?mg/mL, particularly at 20?mg/mL) significantly prevented EtOH-induced cytotoxicity and reduced the increase of ALT, AST, LDH and MDA contents, as well as inhibited the reduction of SOD contents in the HepG2 cells, indicating that CPs protected the cells from oxidative damage induced by EtOH metabolism, and thus had a great application potential in hepatoprotective nutraceuticals. |
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Keywords: | Corn peptides ethanol-induced damage antioxidant activity HepG2 |
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