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透析法精制玉米须多糖工艺的研究
引用本文:周鸿立,李春华,张英俊. 透析法精制玉米须多糖工艺的研究[J]. 上海中医药杂志, 2010, 0(10): 81-83
作者姓名:周鸿立  李春华  张英俊
作者单位:[1]吉林化工学院药学系,吉林吉林132022 [2]中油吉化集团公司总医院药剂科,吉林吉林132022
基金项目:吉林省教育厅“十一五”科学技术研究项目资助(2008-182)
摘    要:目的优化透析法除玉米须多糖中小分子杂质的工艺条件。方法苯酚-硫酸法测定多糖含量。在单因素基础上,通过3因素3水平的正交试验研究透析法除去小分子杂质的工艺,3因素分别为更换缓冲液次数、透析时间和透析温度。结果透析液与缓冲液体积比为1∶50的最佳透析条件为透析时间为7h,温度为30℃,更换缓冲液次数为3次。结论多糖纯度由原来的27.0%提高到42.35%,多糖的平均损失率9.25%。

关 键 词:玉米须多糖  透析法  苯酚-硫酸法

Study on dialysis for refining polysaccharide from stigma maydis
ZHOU Hong-li,LI Chun-hua,ZHANG Ying-jun. Study on dialysis for refining polysaccharide from stigma maydis[J]. Shanghai Journal of Traditional Chinese Medicine, 2010, 0(10): 81-83
Authors:ZHOU Hong-li  LI Chun-hua  ZHANG Ying-jun
Affiliation:1.School of Pharmacy,Jilin Institute of Chemical Technology;2.General Hospital of Jihua Group,PetroChina,Jilin )
Abstract:Objective To optimize the processing conditions of removing small molecule impurities from polysaccharides by dialysis.Methods Based on single factor,the orthogonal experiments of 3 factors and 3 levels were done to remove small molecule impurities;the three factors were the number of replacement buffer,time of dialysis and dialysis temperature.Results The phenol-sulfuric acid method was used to assay polysaccharide content.The best condition of orthogonal experiment was dialysis time for 7 h,the temperature at 30℃,changing buffer frequency for 3(volume ratio of fluid and the buffer is 1∶50).Conclusion Polysaccharide content is increased from 27.0% to 42.35% and loss rate of is 9.25%.
Keywords:Polysaccharide from stigma maydis  dialysis  phenol-sulphuric acid method
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