透析法精制玉米须多糖工艺的研究

目的优化透析法除玉米须多糖中小分子杂质的工艺条件。方法苯酚-硫酸法测定多糖含量。在单因素基础上,通过3因素3水平的正交试验研究透析法除去小分子杂质的工艺,3因素分别为更换缓冲液次数、透析时间和透析温度。结果透析液与缓冲液体积比为1∶50的最佳透析条件为透析时间为7h,温度为30℃,更换缓冲液次数为3次。结论多糖纯度由原来的27.0%提高到42.35%,多糖的平均损失率9.25%。

Study on dialysis for refining polysaccharide from stigma maydis

Objective To optimize the processing conditions of removing small molecule impurities from polysaccharides by dialysis.Methods Based on single factor,the orthogonal experiments of 3 factors and 3 levels were done to remove small molecule impurities;the three factors were the number of replacement buffer,time of dialysis and dialysis temperature.Results The phenol-sulfuric acid method was used to assay polysaccharide content.The best condition of orthogonal experiment was dialysis time for 7 h,the temperature at 30℃,changing buffer frequency for 3（volume ratio of fluid and the buffer is 1∶50）.Conclusion Polysaccharide content is increased from 27.0% to 42.35% and loss rate of is 9.25%.