连翘药材HPLC指纹图谱的研究

目的建立连翘药材HPLC指纹图谱。方法采用HPLC法,用Shiseido Capcell pak MG C18色谱柱（250mm×4.6mm,5μm）,乙腈-0.2%磷酸梯度洗脱,流速为1ml.min-1,检测波长210nm,柱温：25℃。以中药色谱指纹图谱相似度评级系统（版本2004A）软件计算,先建立标准指纹图谱,并与其他产地翘药相似度计算。结果以河南卢氏产连翘建立标准指纹图谱,其他产地连翘药材指纹图谱的相似度在0.704-0.938,确定了12个共有峰,建立了连翘药材HPLC指纹图谱。结论本法可作为连翘药材的质量控制方法。

Study on HPLC fingerprint chromatogram of Forsythia Suspensa （Thunb.） Vahl

Objective To establish the fingerprint for Fructus Forsythiae by HPLC.Methods The analysis was performed on a Shiseido Capcell pak MG C18 column（250 mm × 4.6 mm,5 μm） with acetonitrile-0.2% phosphoric acid in water（gradient elution） as mobile phase,and at a column temperature of 25℃,and the detection wavelength was 210 nm.The results were analyzed by materia medica chromatographic fingerprints similarity rating system.Results The established fingerprint showed that 12 peaks were common in different batches with the material from Henan Lushi as standard fingerprint,and the similarity of other batches were in the range of 0.704-0.938,which met technical standard of fingerprint.Conclusion The method is stable for quality control of Fructus Forsythiae.