女贞子对脊髓损伤大鼠HSP70的表达及自由基的影响

目的：探讨女贞子对脊髓损伤大鼠的神经保护作用。方法：采用改良Allen法造模，随机分为假手术组、模型组和治疗组，每组30只。在造模成功后6个时间点（1h、6h、24h、3天、6天、14天）取出脊髓标本，检测脊髓组织中热休克蛋白70（HSP70）的表达，造模后6h测定脊髓组织中超氧化物歧化酶（SOD）和丙二醛（MDA）的含量。结果：治疗组脊髓组织HSP7。的表达在所有时间点较模型组明显增强（P〈0．05），且表达高峰提前至1天；治疗组于损伤后6h，SOD高于模型组，MDA低于模型组，与模型组比较，差异均有显著性意义（P〈0．05）。结论：女贞子在脊髓损伤旱期有效清除自由基，显著增强损伤部位HSP70的表达，保护脊髓神经组织。

Effect of Fructus Ligustri Lucidi on Heat Shock Protein70 Expression and Free Radicals in Rats After Spinal Cord Injury

Objective:To observe the protective effect of Fructus Ligustri Lucidi on the rats with spinal cord injury.Methods: Rat model of spinal cord injury was established by Allen’s method,and 90 male rats were equally randomized into three groups,sham operation group,model group and treatment group.HSP70 was measured at time point of 1h,6h,24h,3d,6d,and 14d.Spinal SOD and MDA contents were measured at 6h after modeling.Results:In treatment group,the expression of heat shock protein70(HSP70) in spinal cord tissues was obvious enhanced in comparison with model group,and the difference was statistically significant(P < 0.05).The expression arrived at peak at day one.At 6h,SOD in the treatment group was obvi-ously higher and MDA was lower than the model group,and the difference was statistical significant(P < 0.05).Conclusions: Fructus Ligustri Lucidi could protect spinal cord by eliminating free-radicals and increasing expression of HSP70 in rats with early spinal cord injury.