芳香化浊液对流感病毒FM1株体外抑制作用实验研究

目的：观察芳香化浊液体外抑制甲1型流感病毒FM1株的作用。方法：通过在细胞培养液中加入不同浓度的芳香化浊液，并设置阳性药物、正常细胞对照，观察细胞不出现病变的最小稀释浓度作为该药的无毒界限。接种甲1型流感病毒FM1株于狗肾传代细胞（MDCK），采用细胞病变抑制法观察不同浓度的芳香化浊液体外抑制甲1型流感病毒FM1株的作用。结果：芳香化浊液的最大无毒浓度为1：16000，细胞病变抑制法检测结果显示：芳香化浊液在最小无毒浓度1：64000时可表现为对甲1型流感病毒FM1株的完全抑制作用。结论：芳香化浊液体外能明显抑制甲1型流感病毒FM1株，且有效治疗浓度无细胞毒性。

In-vitro Inhibitory Effect of Fangxiang Huazhuo Liquid on Influenza Virus FM1 Strain

Objective:To investigate the inhibitory effect of Fangxiang Huazhuo Liquid(FHL),a Chinese medicinal preparation composed of aromatic herbs and with the action of resolving turbidity on influenza A virus FM1 strain infection.Methods:The cultured MDCKcells were treated with different concentrations of FHL,and positive drug group and normal control group were also set up.The non-toxic limit of the drug was confirmed after observation of the minimal dilution concentration of the drug which could not cause pathological changes.MDCK cells were inoculated with influenza A virus FM1 strain,and cytopathic effect inhibition assay was used to observe the inhibitory effect of different concentrations of FHL on influenza A virus strain FM1 in vitro.Results:FHL of The maximum non-toxic concentration of FHL was 1∶16000,and the results of cytopathic effect inhibition assay showed that FHL at minimum-toxic concentration of 1∶64000 had completely inhibition of influenza A virus FM1 strain in vitro.Conclusion:FHL exerts certain antiviral effect on influenza A virus FM1 strain in vitro.