间期细胞核中abl和bcr基因的三维空间分布在bcr／abl融合基因形成中的作用

目的 从生物体视学角度分析bcr/abl融合基因的形成机制。方法 应用荧光原位杂交和激光共聚焦扫描显微镜技术，观察γ－射线对IM9细胞中的bcr和abl基因在间期核内三维空间分布的影响。结果 abl和bcr基因在间期核内有其特定的分布区域，且该分布区域在细胞周期中呈规律性的动态变化。放射性照射会使abl和bcr基因之间的距离缩短。结论 abl和bcr基因在间期细胞核内的易接近性是bcr/abl融合基因形成的因素之一。

Role of the three-dimensional distribution of abl and bcr genes in the formation of bcr/abl fusion gene in interphase neucleus.]

OBJECTIVE: To explore the mechanism of bcr/abl fusion gene formation in view of its biological stereology. METHODS: Assisted by fluorescent in situ hybridization combined with confocal laser scanning microscopy, we observed the effect of gamma-ray exposure on three-dimensional distribution of bcr and abl genes in the interphase nucleus of IM-9 cell line. RESULTS: In interphase nuclei of IM-9 cells, abl and bcr genes retained their own definite distribution patterns that were dynamically and regularly modulated along with the phases of the cell cycle. gamma-ray exposure, however, produced shortened distances between the 2 genes. CONCLUSION: The accessibility of abl and bcr genes to each other in the interphase nuclei is one of the factors for bcr/acl fusion gene formation.