| 比色法测定参芪含片中总皂苷的含量 |
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| 引用本文： | 骆苏芳,许重远,陈英.比色法测定参芪含片中总皂苷的含量[J].中国医院用药评价与分析,2010(5):448-449. |
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| 作者姓名： | 骆苏芳 许重远 陈英 |
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| 作者单位： | 南方医科大学健康食品研究所;南方医科大学南方医院药材科; |
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| 摘 要： | 目的：测定参芪含片中总皂苷的含量以建立质控方法。方法：采用香草醛-硫酸比色法测定总皂苷的含量。结果：以人参皂苷Re为对照品作校正曲线,在0.025～0.175mg·mL-1范围内线性关系良好。加样回收率为98.65%,RSD为3.9%。测得2批参芪含片中总皂苷含量分别为17.25mg·g-1和17.47mg·g-1。结论：该方法简便、准确、灵敏、重复性好,可作为参芪含片中总皂苷的质控方法。
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| 关 键 词： | 参芪含片 总皂苷 分光光度法 含量测定 |
Determination of Total Saponins in Sengqi Oral Tablets by Colorimetry |
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| LUO Su-fang,XU Chong-yuan,CHEN Ying.Determination of Total Saponins in Sengqi Oral Tablets by Colorimetry[J].Evaluation and Analysis of Drug-Use in Hospital of China,2010(5):448-449. |
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| Authors: | LUO Su-fang XU Chong-yuan CHEN Ying |
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| Institution: | LUO Su-fang1,XU Chong-yuan2,CHEN Ying2(1. Health Food Research Institute,Southern Medical University,Guangzhou 510515,China,2. Dept. of Pharmacy,South Hospital of Southern Medical University,China) |
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| Abstract: | OBJECTIVE： To determine the content of total saponins in Sengqi oral tablets so as to establish the quality control method for this medicine. METHODS： The content of total saponins was determined by citroellal-sulphuric acid colorimetry. RESULTS： With Saponins of panaxsaponin Re as the reference substance,the calibration curve had a good linearity within a concentration range of 0. 025 ～ 0. 175 mg · mL-1 for saponins. The average recovery was 98. 65% and the RSD was 3. 9% . The contents of total saponins in 2 batches Sengqi oral tablets were 17. 25 mg· g-1 and 17. 47 mg · g-1,respectively. CONCLUSION： The established method is simple,accurate, sensitive and reproducible,and it can be used for the quality control of the total saponins in Sengqi oral tablets. |
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| Keywords: | Sengqi oral tablet Total saponins Spectrophotometry Determination |
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