荧光定量PCR法检测呼吸道感染患儿支原体、衣原体感染

目的:了解呼吸道感染患儿肺炎支原体、解脲脲原体、沙眼衣原体的感染情况。方法:应用荧光定量聚合链式反应法对571例呼吸道感染患儿同时进行肺炎支原体、解脲脲原体、沙眼衣原体检测。结果:肺炎支原体、解脲脲原体、沙眼衣原体总检出率为56.0％,其中肺炎支原体为29.1％,解脲脲原体为23.3％,沙眼衣原体为8.2％,肺炎支原体和解脲脲原体为3.2％,解脲脲原体和沙眼衣原体为1.4％。在年龄分布上,<28 d组患儿解脲脲原体感染率高达30.3％,且与其他组的差异有显著性,1个月-1岁组、>1-3岁组和>3岁组的患儿肺炎支原体感染率明显高于<28 d组,差异有显著性。肺炎支原体感染率四季差异无显著性,解脲脲原体冬春感染率高,沙眼衣原体春季感染率低。血和痰两种类型标本,肺炎支原体与解脲脲原体阳性率之间差异有显著性,与沙眼衣原体阳性率之间差异无显著性。结论:新生儿较其他年龄段患儿易感解脲脲原体、沙眼衣原体;肺炎支原体随着年龄的增长感染率逐渐增高。痰标本的阳性率明显高于血液。荧光定量聚合链式反应法诊断支原体、衣原体感染快速、敏感,特异性高。

Investigation on infection of mycoplasma pneumoniae,Ureaplama urealyticum and Chlamydia trachomatis infection in children with respiratory tract infection

ObjectiveTo investigate the situation of infection of mycoplasma pneumoniae(MP) , Ureaplama urealyticum (Uu) and Chlamydia trachomatis (CT) in children with respiratory tract infection. Methods MP,Uu and CT were measured simultaneously by fluorogenic quantitative PCR in 571 children with respiratory tract infection. Results Total infectious rate of MP,Uu and CT was 56.0%, of Mp was 29.1%,of Uu was 23. 3% ,of CT was 8. 2%,of MP and Uu was 3.2%, of Uu and CT was 1. 4%. The group under twenty-eight days old had higher Uu positive (30.3%) than other groups (P>0.05). The infectious rate of MP was not significant different in four seasons(P>0.05), the infectious rate of Uu was higher in winter and spring, the infectious rate of CT was lower in spring. There was significant difference in MP and Uu between serum and sputum samples(P<0. 05). Conclusion Neonates have suscepitility to Uu and CT; infectious rate of MP is increased with ages. Positive rate of sputum speciments is higher than serum. Fluorogenic quantitative PCR is sensitive,specific and rapid to increase the diagnosis of MP,Uu and CT infections.