蒲葵根提取物的体外抗肿瘤实验研究

目的:用体外细胞培养法研究蒲葵根提取物对七种肿瘤细胞株生长的抑制作用。方法:采用MTT法、细胞集落形成法、生长曲线法测定蒲葵根提取物对白血病细胞株L1210和P388D1、宫颈癌细胞株Hela、人胃癌细胞株SGC7901、黑色素瘤细胞株B16、神经性肿瘤细胞株NG108-15、人肝癌细胞株Hele 7404生长的抑制情况。结果:MTT法、细胞集落形成法、生长曲线法测定的结果均显示,当蒲葵根提取物浓度在5.0μg/m l以上时,7类肿瘤细胞株的生长均受到明显抑制。结论:蒲葵根提取物在体外具有抗肿瘤作用。

Study on the Anticancer Effects of Extracts from Roots of Livistona chinensis in Vitro

OBJECTIVE: To observe the effects of extracts from root of Livistona chinensis on the growth inhibition in seven carcinoma cell lines. METHODS: The growth inhibition was analyzed by MTT, cell colony and cell growth curve measuremen technique in the stomach carcinoma SGC7901, Lymphocytic leukemia L1210, Lymphoid neoplasm P388D1, tumor of cervix uteri Hela, hepar carcinoma hele 7404, melanoma B16 and mouse neuroblastomax rat glioma hybrid NG108-15 cells lines. RESULTS: The growth of all tumor cells were inhibited by ethyl acetate of alcohol extract from roots of Livistona chinensis. The growth of all tumor cells were not affected by low dose extracts (0.5 microg/ml). The growth of all tumor cells were obviously inhibited by higher dose extracts (5.0 microg/ml). The growth of all tumor cells were inhibited in growth curve measurement. CONCLUSION: The results show that ethyl acetate of alcohol extracts from roots Livistona chinensis possesses the role of antitumor in cell culture.