HPLC测定肝得治胶囊中原儿茶醛、丹参素含量

目的 高效液相色谱法测定肝得治胶囊中原儿茶醛、丹参素的含量。方法 采用ODSC，柱（5μm，4．6mm×250mm），流动相为甲醇-1％冰醋酸（18：82），检测波长为280mm，流速1．0ml／min，柱温：室温。结果 原儿茶醛在2．9～58μg／ml之间线性良好，γ=0．9999；丹参素在7．1～142μs／ml之间线性良好，γ=0．9999；原儿茶醛平均回收率100．3％，RSD=3．4％（n=6），丹参素平均回收率99．5％。RSD=1．9％（n=6）。结论 该方法能准确可靠地同时测定肝得治胶囊中原儿茶醛、丹参素含量，能够有效地控制该制剂的质量。

HPLC determination of protocatechualdehyde and Danshensu in gandezhi capsule

Objective To establish a HPLC method for the determination of protocatechualdehyde and Danshensu in Gandezhi capsule. Methods HPLC method was adopted with ODS C18 （4. 6 mm×250mm,5 m） at room temperature. The mobile phase consisted of methanol-0.1% acetic acid solution （ 18 ： 82） at a flow rate of 1.0 ml/min. A UV-detector at 280 nm was used. Results The linear relation of protocatechualdehyde and Danshensu were excellent within the range of 2.9 - 58 μg/ml （ r = 0. 999 9 ） and 7.1 - 142 μg/ml （ r = 0. 999 9） respectively. The average recoveries were 100.3% and 99. 5% respectively with RSD at 3.4% （n = 6） and 1.9% （ n =6） respectively. Conclusion The method is simple,rapid,accurate,sensitive and reproducible,and suitable for the quality control of Gandezhi capsule.