miR-34a通过Notch信号通路影响乳腺癌细胞的生物学行为

目的探讨miR-34a通过Notch信号通路影响乳腺癌细胞的生物学行为。方法乳腺癌细胞MCF-7分为miR-34a及Control组,分别利用脂质体转染miR-34a mimics及miR-34a NC,RT-PCR法检测细胞中miR-34a表达,MTT、Hoechst染色及transwell法分别检测细胞活力、凋亡及侵袭情况;western blot检测细胞中Notch1,Notch2,Dll1及Jagged-1蛋白表达情况。结果与Control组比较,miR-34 mimics组中miR-34a表达量上调,细胞皱缩,变圆变亮,细胞活力降低(P0.01),细胞凋亡率显著提高(P0.01),细胞侵袭数目减少(P0.01),Notch1,Notch2,Dll1及Jagged-1蛋白表达量都显著降低(P0.01)。结论 miR-34a mimics通过抑制Notch信号通路进而诱导MCF-7乳腺癌细胞凋亡并抑制其侵袭。

Effect of miR-34a on the biological behavior of breast cancer cell by Notch signal pathway

Objective To explore effect of miR-34a on the biological behavior of breast cancer cell MCF-7 by Notch signal pathway. Methods The MCF-7 cells were divided into miR-34a and Control groups. The miR-34a mimics and miR-34a NC were transfected by liposome. The expression of miR-34a in MCF-7 cell was examed by RT-PCR. The cell viability was detected by MTT. The cell apoptosis was examed by Hoechst staining. The cell invasion was detected by transwell method. The expression of Nothch1, Nothc2, Dll1 and Jagged-1 was detected by western blot. Results Compared with Control group, the expression of miR-34a was up-regulated (P<0.01), Cell shrinkage, rounded brighten,the cell viability was decreased (P<0.01), cell apoptotic rate was increased (P<0.01), cell invasion number was reduced (P<0.01), the expression of Notch1, Nothc2, Dll1 and Jagged-1 was down-regulated (P<0.01). Conclusion miR-34a mimics induced MCF-7 cell apoptosis and inhibited cell invasion via inhibition of Notch signal pathway.