双黄连注射液及其中间品对RBL-2H3细胞增殖的影响

目的:探讨双黄连注射液(SHLI)及其中间品(金银花连翘提取物、黄芩提取物)不同浓度对白血病细胞株RBL-2H3增殖和形态的影响,为类过敏细胞模型的建立及药品质量的监控提供实验依据。方法:将双黄连注射液以体积比1∶16为初始浓度,按2倍梯度稀释至1∶4 096,中间品分别以体积比1∶8为初始浓度,按2倍梯度稀释至1∶2 048,作为受试样品。以各受试样品分别孵育RBL-2H3细胞,观察细胞形态变化。应用噻唑蓝(MTT)比色法测定各组RBL-2H3细胞增殖活力,并计算细胞增殖的半数抑制浓度(IC50)。根据IC50设置不同给药浓度,观察SHLI对RBL-2H3细胞凋亡的影响。结果:随着给药浓度的升高,SHLI及其中间品对细胞增殖的抑制作用逐渐增强,细胞存活力明显降低。给药后随着药物浓度的升高,细胞损伤逐渐加重,形态发生明显变化。高浓度SHLI可引起RBL-2H3细胞凋亡。结论:SHLI可抑制RBL-2H3细胞增殖活力,为SHLI类过敏细胞模型的建立,评价SHLI及中间品的安全性及药物质量控制提供依据。

Effect of Shuanghuanglian Injection and Its Intermediates on Proliferation of RBL-2H3 Cells

Objective: To observe the effect of different concentrations of Shuanghuanglian injection (SHLI) and its intermediates on proliferation of RBL-2H3 cells, and provide evidence for establishing anaphylactoid cell models and monitoring drug quality. Method: RBL-2H3 cells were treated with different concentrations of SHLI (2 times gradient dilution from volume ratio 1: 16 to 1: 4 096) and its intermediates (2 times gradient dilution from volume ratio 1: 8 to 1: 2 048). The cellular morphology was observed under inverted microscope. MTT colorimetric assay was used to determine the growth of RBL-2H3 cells in different concentration of SHLI and its intermediates, and the half inhibitory concentration (IC₅₀) was calculated. Dependent on the value of IC₅₀, RBL-2H3 cells were then treated with different concentrations of SHLI to observe the effect of SHLI on cells apoptosis. Result: As the concentration increases of SHLI and its intermediates, their inhibitory effect on proliferation of RBL-2H3 cells was gradually increased; the cells survival rate was decreased significantly; cells injury was gradually aggravated; and the cellular morphology was also changed. High dose of SHLI could induce apoptosis of RBL-2H3 cells. Conclusion: SHLI and its intermediates could reduce RBL-2H3 cells proliferation, we can provide certain experiment basis for establishing anaphylactoid cell models and monitoring drug quality and safety.