胡枝子提取物对子宫肌瘤模型动物及血瘀大鼠的影响

目的:探索胡枝子提取物(LBE)对子宫肌瘤模型动物及血瘀大鼠的影响。方法:取同日龄KM小鼠及同日龄SD大鼠,分成2组,分别为正常组,造模组,造模组制作苯甲酸雌二醇采用子宫肌瘤大、小鼠模型,将造模大鼠组随机分为模型组,阳性药组枸橼酸他莫昔芬片(0.18 g·kg-1),LBE高、中、低剂量组(1.0,0.5,0.25 g·kg-1),将造模组小鼠随机分为模型组,阳性组枸橼酸他莫昔芬片(0.23 g·kg-1),LBE高、中、低剂量组(1.4,0.7,0.35 g·kg-1);将大鼠随机分6组,分别为正常组,模型组,阳性药复方丹参片组(0.34 g·kg-1),LBE高、中、低剂量组(1.0,0.5,0.25 g·kg-1),除正常组外,其余各组大鼠背部ih给予盐酸肾上腺素2 h后将大鼠浸浴于冰水中,造成急性血瘀大鼠模型,连续给药2周,观察子宫肌瘤模型动物的子宫系数、子宫干重、血清雌激素水平及子宫病理形态学变化和血瘀大鼠的血流变指标。结果:连续给药2周,LBE组子宫肌瘤大、小鼠的子宫系数、子宫干重、血清雌激素水平降低,子宫肌瘤小鼠病理形态学变化改善;血瘀大鼠全血黏度、血浆黏度、全血高切相对指数、全血低切相对指数、红细胞聚集指数降低,与模型组比较均具有统计学差异(P0.01,P0.05)。结论:LBE对子宫肌瘤动物模型具有一定的治疗作用。

Effect of Lespedeza Bicoloris Radix Extract on Animal Models of Uterine Fibroidand Blood Stasis Rats

Objective: To explore the effect of Lespedeza Bicoloris Radix extracts (LBE) on animal models of uterine fibroid and blood stasis rats. Method: KM mice of the same birth date and SD rats of the same birth date were divided into normal groups and model groups. Estradiol benzoate was used in modeling animals to induce uterine fibroid rat models and uterine fibroid mice models. The modeling rat groups were randomly divided into model group, Tamoxifen citrate tablets positive group (0.18 g · kg^-1) and LBE high dose, middle dose and low dose groups (1.0, 0.5, 0.25 g · kg^-1). The modeling mice were randomly divided into model group, Tamoxifen citrate tablets positive group (0.23 g · kg^-1) and LBE high dose, middle dose and low dose groups (1.4, 0.7, 0.35 g · kg^-1). The rats were randomly divided into normal group, model group, Compound Danshen tablets positive group (0.34 g · kg^-1) and LBE high dose, middle dose and low dose groups (1.0, 0.5, 0.25 g · kg^-1). All the other rats except normal group received subcutaneous injection of adrenaline hydrochloride in back. 2 h later, rats received dipping bath in ice water to induce acute blood stasis mice models. After continuous drug administration for two weeks, uterus coefficient, uterine dry weight, serum estrogen level, and pathological changes of uterus in uterine fibroid animal models and blood rheology in blood stasis rats were observed. Result: After continuous administration for two weeks, uterus coefficient, uterine dry weight, and serum estrogen levels were decreased in uterine fibroid rats and mice LBE groups, and the pathological changes were alleviated in uterine fibroid mice;whole blood viscosity, plasma viscosity, whole blood high shear relative index, whole blood low shear relative index, and erythrocyte aggregation index were decreased in blood stasis rats (P<0.01, P<0.05). Conclusion: LBE has a certain therapeutic effect on animal models of uterine fibroid.