| 高效液相色谱-蒸发光散射检测器法测定黄芪健胃膏中黄芪甲苷的含量 |
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| 引用本文： | 王伟兰,孙艳. 高效液相色谱-蒸发光散射检测器法测定黄芪健胃膏中黄芪甲苷的含量[J]. 解放军药学学报, 2009, 25(2): 180-181 |
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| 作者姓名： | 王伟兰 孙艳 |
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| 作者单位： | 解放军总医院,药品保障中心,北京,100853;解放军总医院,药品保障中心,北京,100853 |
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| 摘 要： | 目的建立黄芪健胃膏中黄芪甲苷的含量测定方法。方法采用蒸发光散射检测器对黄芪健胃膏中的黄芪甲苷进行HPLC分析。结果黄芪甲苷在1．2-14．0μg范围内，具有良好的线性关系，r=0．9997，平均加样回收率为98．7％，RSD为0．98％。结论本文所建立的HPLC法简便，可靠，易行，适用于黄芪健胃膏的质量控制。
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| 关 键 词： | HPLC 蒸发光散射检测器 黄芪健胃膏 黄芪甲苷 |
Determination of Astragaloside IV in HuangQi JianWei Soft Extract by HPLC-ELSD |
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| WANG Wei-Lan,SUN Yan. Determination of Astragaloside IV in HuangQi JianWei Soft Extract by HPLC-ELSD[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2009, 25(2): 180-181 |
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| Authors: | WANG Wei-Lan SUN Yan |
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| Affiliation: | 1The Pharmacy Center of Chinese PLA General Hospital, Beijing 100853 China) |
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| Abstract: | Aim To determine astragaloside Ⅳ in HuangQi JianWei Soft Extract by HPLC. Methods HPLC and a detector （ELSD） were used in the quantitative analysis of astragaloside Ⅳ in HuangQi JianWei Soft Extract. Results The linear range of peoniflorin was 1.2 - 14.0μg, r = 0. 999 7. The average recovery of peoniflorin was 98.7% and RSD was 0.98%. Conclusion The method is simple, reliable, and good for determination of astragaloside Ⅳ in HuangQi JianWei Soft Extract. |
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| Keywords: | HPLC |
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