Evaluation of the new test VERSANT CT/GC DNA 1.0 assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens |
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Authors: | Marangoni Antonella Foschi Claudio Nardini Paola D'Antuono Antonietta Banzola Nicoletta Di Francesco Antonietta Cevenini Roberto |
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Institution: | Section of Microbiology, DESOS, University of Bologna, Bologna, Italy. antonella.marangoni@unibo.it |
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Abstract: | Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) are the two most common sexually transmitted bacterial infections in developed countries. The purpose of the present study was evaluating a new system for CT/GC detection in urine specimens. A total of 700 urine specimens were obtained from patients attending the STD Outpatients Clinic of St. Orsola University Hospital, Bologna, Italy. Samples were tested by VERSANT? CT/GC DNA 1.0 Assay (Siemens Healthcare Diagnostics Inc., Tarrytown, NY), a multiplex Real-Time PCR assay, for simultaneous CT/GC detection. Results obtained by VERSANT assay were compared with those obtained by culturing genital secretions of the same patients. Moreover, urine specimens testing positive in VERSANT assay were retested by in-house PCR assays, used as confirmatory tests. VERSANT? CT/GC DNA 1.0 Assay performed with 99.4% and 99.2% of specificity for GC and CT detection, respectively, whereas sensitivity was 100% both for CT and GC. Culture methods were 100% specific, but far less sensitive than VERSANT assay. VERSANT? CT/GC DNA 1.0 Assay demonstrated to be a highly sensitive and specific technique for CT/GC detection. |
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Keywords: | sexually transmitted infections nucleic acids amplification techniques urine samples omp1 PCR cppB PCR |
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