Evaluation of the wound healing potential of Wedelia trilobata (L.) leaves |
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Authors: | Balekar Neelam Katkam Nadpi Gangadhar Nakpheng Titpawan Jehtae Kholeeyoh Srichana Teerapol |
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Institution: | Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. |
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Abstract: | Ethnopharmacological relevanceWedelia trilobata (L.) Hitchc (Asteraceae) leaves are used in the treatment of wounds by traditional healers. Despite the use of this plant in wound healing, there is a scarcity of scientific data to support its therapeutic application.Aim of the studyTo investigate the wound healing potential of Wedelia trilobata (L.) leaves commonly employed by traditional healers and to clarify its traditional use in a scientific investigation.Materials and methodsAn ethanolic extract of Wedelia trilobata leaves was subjected to column chromatography. Hexane, ethyl acetate (WEA) and chloroform:methanol (50:50) (WCM) fractions were obtained. The fractions were tested using relevant in vitro wound healing assays. Antioxidant activity was measured by the DPPH assay. The fibroblast proliferation, oxidative stress using hydrogen peroxide, an in vitro scratch assay, and increasing collagen content was determined using fibroblast L929. Minimum inhibitory concentrations (MICs) were determined against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa.ResultsWEA (3 μg/mL) promoted fibroblast L929 survivability up to more than 90% before and more than 85% after hydrogen peroxide induced oxidative stress. WEA (3 μg/mL) induced a 70% migration rate in the in vitro scratch assay and the collagen content was increased to 261 μg/mL compared to the control (57.5 μg/mL). WCM exhibited a scavenging activity for DPPH with an IC50 value of 179.5 μg/mL comparable to BHT (139.3 μg/mL). WEA was active against Gram positive bacteria Staphylococcus aureus, Staphylococcus epidermidis with MIC values of 62.5 and 31.25 μg/mL, respectively.ConclusionThese scientific findings of wound healing activity supports the traditional claims for Wedelia trilobata (L.) leaves. The WEA displayed antibacterial and fibroblast stimulatory activities while WCM exhibited antioxidant to indicate its potential wound healing properties. However further studies to isolate the antibacterial, antioxidant and fibroblast stimulatory compounds that contribute to the wound healing properties of this plant are needed. |
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Keywords: | BHT butylated hydroxy toluene CFU colony forming unit DMEM Dulbecco's Modified Eagle Medium DMSO dimethyl sulfoxide DPPH 11-diphenyl-2-picrylhydrazyl E coli Escherichia coli EDTA ethylene diamine tetraacetic acid disodium salt FBS fetal bovine serum H2O2 hydrogen peroxide MBC minimum bactericidal concentration MIC minimum inhibitory concentration MTT 3-(4 5-dimethylthiazolyl)-2 5-diphenyltetrazolium bromide P aeruginosa Pseudomonas aeruginosa ROS reactive oxygen species S aureus Staphylococcus aureus S epidermidis Staphylococcus epidermidis SD standard deviation |
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