上海市浦东地区淋病奈瑟菌分型与耐药性的相关分析

目的了解上海市浦东地区淋病病原体奈瑟双球菌的不同基因分型及各基因型与耐药性之间的对应关系。方法运用随机扩增多态性DNA(RAPD)指纹图谱对78株分离自不同患者淋球菌菌株进行区分，从分子水平对淋球菌进行基因分型，并在此基础上探讨不同的基因分型与耐药性之间的关系。结果78株淋球菌分离株RAPD图谱上相似，但各菌株基因图谱之间有明显不同DNA多态性，可将菌株区分为Ⅰ、Ⅱ、Ⅲ三种基因分型，对此三种基因分型与A、B、C、D四种不同的耐药类型进行对应分析，发现耐药类型与基因型别之间存在对应关系。结论通过研究发现浦东地区淋球菌流行株有着不同的耐药特点及基因型别，耐药性与不同的基因分型之间存在着一定相关性。

Correspondence analysis on random amplified polymorphic DNA genotyping and drug-resistance of neisseria gonorrhoeae strains in Pudong area, Shanghai

OBJECTIVE: Using molecular epidemiology methods to investigate relationship between genotypes and drug-resistance of neisseria (N.) gonorrhoeae in Shanghai area. METHODS: A random amplified polymorphic DNA (RAPD) fingerprint method at the molecular level was used to differentiate the strains which were isolated from the outpatients of sexually transmitted disease clinics. The sensitivity to antibiotic of the 78 N. gonorrhoeae strains on 9 different antibiotics was tested and the relationship between different genotypes and phenotypes was studied. RESULTS: Selected RAPD primer could give out a group of amplification polymerase chain reaction bands with some main segments common to all the N. gonorrhoeae strains tested and some segments were different among the N. gonorrhoeae strains. All the 78 N. gonorrhoeae strains could be classified as three different groups (I, II and III). The strains could also be distinguished as four types (A, B, C and D) according to drug-resistance status. Using correspondence analysis method, the relationship between the three genotypes and four resistance types could be identified. CONCLUSION: RAPD fingerprint seemed a useful genotyping method and could be used for molecular epidemiological studies.