| 高效液相色谱-电喷雾串联四极杆质谱法研究大鼠尿内毛果芸香碱代谢产物 |
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| 引用本文： | 张喆,齐影.高效液相色谱-电喷雾串联四极杆质谱法研究大鼠尿内毛果芸香碱代谢产物[J].医药世界,2006(9):53-54. |
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| 作者姓名： | 张喆 齐影 |
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| 作者单位： | 1. 长春百克药业有限责任公司,130023
2. 长春生物制品研究所,130023 |
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| 摘 要： | 首次应用高效液相色谱-电喷雾串联四极杆质谱法鉴定了大鼠灌胃给予毛果芸香碱0.2mg后0-8小时内尿中的代谢物。4只大鼠给药毛果芸香碱盐酸盐,收集给药后尿液,固相萃取柱富集、然后应用高效液相色谱-电喷雾串联四极杆质谱法在线进行多反应监测,进而对尿中微量的代谢物进行了质谱解析。分别定性为毛果芸香碱的葡萄糖醛酸结合物、毛果芸香酸和毛果芸香酸的葡萄糖醛酸结合物。而且发现了各种代谢产物及原形药物在大鼠体内均有构型变化。
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| 关 键 词： | 毛果芸香碱 代谢物 液相色谱-串联质谱法 |
| 文章编号： | 1008-6919（2006）09-0053-02 |
Study on metabolites of pilocarpine in rat urine using high-performance Hquid chromatography with tandem mass spectrometry |
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| Abstract: | Metabolism of pilocarpine in rat urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 0. 1 mg. The urine sample was prepared by solid phase extraction, and then was analyzed using multiple reaction monitoring. Five metabolites were identified as oxidative metabolite pilocarpic acid and the glucuronides of pilocarpine and pilocarpic acid. |
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| Keywords: | pilocarpine metabolite liquid chromatography-tandem mass spectrometry |
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