N-myc下游调节基因-1、p53和血管内皮生长因子在食管鳞状细胞癌组织中表达的意义

 【摘要】　目的　探讨N-myc下游调节基因-1（NDRG1）、p53和血管内皮生长因子（VEGF）在食管鳞状细胞癌中的表达及其在食管鳞状细胞癌发生、发展中的意义。方法　采用免疫组织化学SP法检测20例正常食管组织和78例食管鳞状细胞癌组织中NDRG1、p53及VEGF蛋白的表达情况。结果　在正常食管组织、食管鳞状细胞癌组织中NDRG1蛋白阳性表达率分别为100.0 %（20／20）、55.1 %（43／78），p53阳性表达率分别为0（0／20）、65.4 %（51／78），VEGF阳性表达率分别为30.0 %（6／20）、67.9 %（53／78），差异均具有统计学意义（P＜0.01）。在食管鳞状细胞癌组织中NDRG1蛋白的表达与淋巴结转移情况呈负相关（r＝-0.237，P＝0.036），而与食管鳞状细胞癌患者的年龄、性别、肿瘤的分化程度、TNM分期及5年生存率无相关性。Spearman相关系数分析显示p53与NDRG1蛋白表达呈负相关（r＝-0.331，P＝0.003），VEGF与NDRG1蛋白的表达也呈负相关（r＝-0.288，P＝0.011）。结论　NDRG1可能为一个新的抑癌基因，在食管鳞状细胞癌的发生、发展及转移过程中发挥着重要的作用。

Expression and significance of NDRG1, p53 and VEGF in esophageal squamous cell carcinoma

Objective To study the expression and significance of NDRG1 (N-myc downstream regulated gene-1), p53 and VEGF in esophageal squamous cell carcinoma (ESCC). Methods NDRG1, p53 and VEGF protein were detected by immunohistochemistry (IHC, SP method) in 20 cases of normal esophageal squamous epithelium and 78 cases of ESCC. Results The results of IHC shows that in normal esophageal squamous epithelium and esophageal squamous cell carcinoma, the positive rate of NDRG1 was 100.0 ％(20/20) and 55.1％ (43/78) respectively, p53 was 0 (0/20) and 65.4 ％ (51/78) respectively, VEGF was 30.0 ％(6/20) and 67.9 ％ (53/78) respectively, all had statistical significance. There was inverse correlation of NDRG1 expression and lymphatic invasion (r =-0.237, P = 0.036). However expression of NDRG1 was no statistical significance with patient' s age, gender, grade, TNM stage, patient' s five year survival. The expression of p53 was inverse correlated with NDRG1, and the expression of VEGF was inverse correlated with NDRG1 (r =-0.331, P = 0.003). Conclusion NDRG1 may be a new tumor suppress gene and play an important role in the development and metastasis of ESCC.