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竹叶兰中联苄类化学成分和抗肿瘤活性研究
引用本文:刘美凤,吕浩然,丁怡. 竹叶兰中联苄类化学成分和抗肿瘤活性研究[J]. 中国中药杂志, 2012, 37(1): 66-70
作者姓名:刘美凤  吕浩然  丁怡
作者单位:1. 华南理工大学化学与化工学院制药工程系,广州510640;华南理工大学广东省绿色化学产品技术重点实验室,广州510640
2. 华南理工大学化学与化工学院制药工程系,广州,510640
3. 清华大学生命科学学院,北京,100084
摘    要:目的:进行竹叶兰干燥根茎中的化学成分和体外抗肿瘤活性研究。方法:乙醇提取,硅胶、SephadexLH-20及ODS柱色谱分离,采用UV,NMR和MS等方法确定化合物的结构。采用MTT法测试化合物对人肝癌细胞Bel-7402和人胃癌细胞BGC-823抗肿瘤药理活性。结果:分得6个化合物,分别为2,7-二羟基-1-(对-羟基苄基)-4-甲氧基-9,10-二氢菲(2,7-di-hydroxy-1-(p-hydroxylbenzyl)-4-methoxy-9,10-dihydrophenanthrene,1),4,7-二羟基-1-(对-羟基苄基)-2-甲氧基-9,10-二氢菲[4,7-dihydroxy-1-(p-hydroxylbenzyl)-2-methoxy-9,10-dihydrophenanthrene,2],3,3’-二羟基-5-甲氧基-联苄(3,3’-dihydroxy-5-me-thoxybibenzyl,3),反式阿魏酸二十四烷酯[(2E)-2-propenoic acid,3-(4-hydroxy-3-methoxyphenyl)-tetracosyl ester,4],反式阿魏酸二十五烷酯[(2E)-2-propenoic acid,3-(4-hydroxy-3-methoxyphenyl)-pentacosyl ester,5],十五烷酸(pentadecyl acid,6)。体外抗肿瘤实验表明,联苄类化合物具有一定的抗肿瘤活性。结论:除化合物3外,另5个化合物均为首次从竹叶兰中分离得到,联苄类化合物均表现出一定的体外细胞毒活性,开环型联苄类化合物3的抗肿瘤活性强于闭环型联苄类化合物1和2。

关 键 词:竹叶兰  联苄  化学成分  抗肿瘤活性
收稿时间:2011-04-18

Antitumoral bibenzyl derivatives from tuber of Arundina graminifolia
LIU Meifeng,LV Haoran and DING Yi. Antitumoral bibenzyl derivatives from tuber of Arundina graminifolia[J]. China Journal of Chinese Materia Medica, 2012, 37(1): 66-70
Authors:LIU Meifeng  LV Haoran  DING Yi
Affiliation:School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China;Guangdong Provincial Key Lab for Green Chemical Product Technology, Guangzhou 510640, China;School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China;School of Life Sciences, Tsinghua University, Beijing 100084, China
Abstract:Objective: To isolate the bibenzyl derivatives from the tuber of Arundina graminifolia and evaluate the anti-tumor activity of these compounds in vitro. Method: The constituents have been extracted by 95% alcohol and then isolated by column chromatography on silica gel and Sephedax LH-20. The structures were determined by UV, IR, NMR and MS spectral analysis. Result: Six constituents have been isolated, and their structures have been established as 2,7-dihydroxy-1-(p-hydroxylbenzyl)-4-methoxy-9,10-dihydrophenanthrene ( 1 ), 4,7-dihydroxy-1- (p-hydroxylbenzyl)-2-methoxy-9,10-dihydrophenanthrene ( 2 ), 3,3'-dihydroxy-5-methoxybibenzyl ( 3 ), (2E)-2- propenoic acid-3-(4-hydroxy-3-methoxyphenyl)-tetracosyl ester ( 4 ), (2E) -2-propenoic acid-3- (4-hydroxy-3- methoxyphenyl)-pentacosyl ester ( 5 ) and pentadecyl acid ( 6 ), respectively. Conclusion: All compounds except for 3 were isolated from the tuber of A. graminifolia for the first time. Compound 3 with bibenzyl ring opening exhibits stronger anti-tumor activity than that of compounds 1 and 2 with bibenzyl ring closing.
Keywords:Arundina gramnifolia  bibenzyl derivatives  chemical constituents  anti-tumor activities
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