| 雷公藤甲素对诱导CD4+、CD8+T细胞凋亡的作用 |
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| 引用本文： | 林科雄,王长征,钱桂生.雷公藤甲素对诱导CD4+、CD8+T细胞凋亡的作用[J].免疫学杂志,2000,16(1):24-26. |
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| 作者姓名： | 林科雄 王长征 钱桂生 |
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| 作者单位： | 第三军医大学新桥医院呼吸内科研完所,重庆,400037 |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 9870 94 6) |
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| 摘 要： | 目的 探讨雷公藤甲素对ＣＤ４＾＋、ＣＤ８＾＋Ｔ细胞凋亡的作用。方法 分离后的正常人外周血ＣＤ４＾＋、ＣＤ８＾＋Ｔ细胞在外培养条件下，加入雷公藤甲素处理，然后用３末端脱氧核苷酸基（ＴｄＴ）介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）检测凋亡细胞。结果 雷公藤甲素处理后ＣＤ４和ＣＤ８＾＋细胞凋亡率比对照组显著增加，但ＣＤ４＾＋、ＣＤ８＾＋Ｔ细胞凋亡率则针显著差别。结论 雷公藤甲素诱导诱活化的ＣＤ４＾＋、Ｃ
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| 关 键 词： | T细胞 雷公藤 细胞凋亡 雷公藤甲素 |
Effects of triptolide on apoptosis of CD4+ and CD8+ T cells |
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| LIN Ke xiong,WANG Chang zheng,QIAN Gui sheng.Effects of triptolide on apoptosis of CD4+ and CD8+ T cells[J].Immunological Journal,2000,16(1):24-26. |
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| Authors: | LIN Ke xiong WANG Chang zheng QIAN Gui sheng |
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| Abstract: | ObjectiveTo investigate the effects of triptolide on apoptosis of CD4 and CD8 T lymphocytes. MethodsPurified CD4 and CD8 T cells were cultured in the presence or absence of triptolide for 12 hours.The apoptotic cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).ResultsThe apoptotic rates of CD4 and CD8 T lymphocytes treated with triptolide were significantly higher than that treated with ConA alone ( P <0 01),but the apoptotic rates between CD4 and CD8 T cells had no difference ( P >0.05).ConclusionThe immunosupression roles of triptolide are associated with nonselective induction of apoptosis of activated CD4 and CD8 T cells. |
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| Keywords: | T cells apoptosis triptolide |
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