| HPLC—ECD法分析中枢单胺类递质及其在免疫应答过程中的动态变化 |
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| 引用本文： | 杨竞平,杨贵贞.HPLC—ECD法分析中枢单胺类递质及其在免疫应答过程中的动态变化[J].中国免疫学杂志,1990,6(6):370-373,382. |
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| 作者姓名： | 杨竞平 杨贵贞 |
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| 作者单位： | 白求恩医科大学免疫学教研室，白求恩医科大学免疫学教研室 长春 130021，长春 130021 |
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| 摘 要： | 建立快速、简便反相离子对高效液相色谱—电化学检测分析法,一次同时测定生物样品中单胺类递质(NE.E.DA、5—HT)及其前体氨基酸(Tyr、Trp)和主要代谢产物(HVA、5-HIAA共8种化合物。应用该法检测了SRBC免疫小鼠大脑皮质、间脑、脑干8种单胺类化合物的变化规律。免疫后第3天,皮质DA/HVA、间脑NE含量显著降低(P<0.05);第5天,间脑5-HT和脑干5-HIAA含量明显升高(P<0.05)。支持免疫应答可以影响中枢单胺类递质代谢的推论。
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| 关 键 词： | 单胺类递质 HPLC ECD 免疫应答 |
HPLC-ECD assay central monoamine transmitters and kinetic change of central monoamine transmitters during immunological response |
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| Yang Jingping,Yang Guizhen. Immunology.HPLC-ECD assay central monoamine transmitters and kinetic change of central monoamine transmitters during immunological response[J].Chinese Journal of Immunology,1990,6(6):370-373,382. |
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| Authors: | Yang Jingping Yang Guizhen Immunology |
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| Institution: | Yang Jingping,Yang Guizhen. Immunology Department of Norman Bethune University of Medical Sceince,Changchun 130061,China |
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| Abstract: | A rapid and simple chromatographic procedure using HPLC-ECD is described for simultaneousdetermination of NE. E. DA. 5-HT with their precursor amino acid (Tyr. Trp) and their main metabolites (HVA. 5-HIAA). Using this assay,eight substrates are measured in cortex, diencephalon and brain stem of mice duing immunological response challenged by SRB6 3 days after challenged, the DA HVA in cortex, NE in diencephalon decrease obviously (p<0.05), and 5 days after challenged, 5-HT in diencephalon and 5-HIAA in brain stem increase significantly (p<0.05), which supports the hypothesis that immunological response may act on metabolism of central monoamine transmitters. |
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| Keywords: | Reverse phase ion-pair HPLC Electrochemical detection Monoamine transmitters Immunological response |
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