Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum |
| |
Authors: | Hui Jin Jin-Ming Lin Xu Wang Tian-bing Xin Shu-xuan Liang Zhen-jia Li Guo-mao Hu |
| |
Institution: | aThe Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Haidian, Beijing 100084, China;bCollege of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;cBeijing Chemclin Biotech Co. Ltd., Beijing 100094, China |
| |
Abstract: | A magnetic particles-based chemiluminescence enzyme immunoassay with high sensitivity, specificity, rapidity, and reproducibility was developed for the determination of free thyroxine in human serum. A competitive assay has been proposed with horseradish peroxidase labeled thyroxine analog. The immunomagnetic particles coated with anti-fluorescein isothiocyanate antibody was used as dispersed solid phase and separation means for the immunoassay. Experimental conditions, such as temperature, the volume of magnetic particles and substrate, incubation time, dilution ratio and other relevant variables upon the immunoassay have been examined and optimized. The proposed method exhibited high performance which the linear range was 1.59–122 pmol L−1 and the detection limit was 0.25 pmol L−1. A coefficient of variance of less than 15% was obtained for both intra-assay and inter-assay precision. The present method has been successfully applied to the analysis of free thyroxine in human serum. The diagnostic accordance rate of the method for normal serum, hyperthyroidism and hypothyroidism are satisfactory. Good correlations were obtained between the results by the proposed method and the commercial radioimmunoassay kit. The present method exhibits good potential in the fabrication of FT4 diagnostic kits which could be used in the clinical analysis and facilitated the development of automated operation systems in the clinical practice. |
| |
Keywords: | Free thyroxine Magnetic particles Chemiluminescence enzyme immunoassay Horseradish peroxidase Human serum |
本文献已被 ScienceDirect 等数据库收录! |
|