首页 | 本学科首页   官方微博 | 高级检索  
     

双重血流同时开放对大鼠肝移植胆管TNF-α表达的影响
引用本文:张毅,冉艳,叶启发. 双重血流同时开放对大鼠肝移植胆管TNF-α表达的影响[J]. 中国医师杂志, 2008, 10(3): 294-296
作者姓名:张毅  冉艳  叶启发
作者单位:1. 贵州省人民医院肝胆外科,贵州,贵阳,550002
2. 贵州省人民医院麻醉科,贵州,贵阳,550002
3. 中南大学湘雅三医院湘雅移植医学研究院
基金项目:贵州省卫生厅科研项目 
摘    要:目的探讨门静脉、肝动脉双重血流同时开放对大鼠肝移植胆道缺血/再灌注(I/R)损伤中的TNF-α表达的影响。方法选用雄性SD大鼠建立大鼠自体原位肝移植模型,随机分为双重血流同时开放组(P组)、门静脉先开放组(N组)和假手术对照组(S组)。供肝再灌注后检测血清ALT、AST、GGT、AKP、TBiL及DBiL水平,比色法测定髓过氧化物酶(MPO)含量,RT-PCR法检测胆管组织TNF-α mRNA表达。结果肝脏再灌注后6h及24h两个时点P组的GGT水平明显低于N组水平(P〈0.05);肝脏再灌注后24h,P组的AKP、TBiL、DBiL水平及胆管损伤病理学评分明显低于N组水平(P〈0.05);再灌注后6h,N组大鼠肝组织的MPO含量明显高于P组(P〈0.05);供肝再灌注后2h、6h,P组大鼠肝组织TNF-α mRNA的相对表达水平明显低于N组(P〈0.05)。结论门静脉、肝动脉双重血流同时开放,有利于减轻肝移植物胆管组织的I/R损伤;其机制可能与TNF-α表达水平的降低以及中性粒细胞(PMN)浸润的减少有关。

关 键 词:血液动力学过程  肝移植  肿瘤坏死因子α

The effects of simultaneous revascularization on the expression of TNF-α during bile duct ischemia-reperfusion injury in rats liver transplantation
ZHANG Yi,RAN Yan,YE Qi-fa. The effects of simultaneous revascularization on the expression of TNF-α during bile duct ischemia-reperfusion injury in rats liver transplantation[J]. Journal of Chinese Physician, 2008, 10(3): 294-296
Authors:ZHANG Yi  RAN Yan  YE Qi-fa
Affiliation:ZHANG Yi, RAN Yan, YE Qi-fa(Department of General Surgery, the Guizhou Provincial People's Hospital, Guiyang 550002 ,China)
Abstract:Objective To investigate the effects of simultaneous hepatic artery and portal revaseularization on the expression of TNF-α during bile duet ischemia-reperfusion injury in rats liver transplantation.Methods Male Spragne-Dawley rats were used to establish an autologous orthotopic liver transphmtation model.Model rats were random divided two groups,simultaneous revascularization group(sroup P)and portal vein revascularization group(group N).The animals were separately killed at the 2nd hour,6th hour and 24th hour after reperfusion.Plasma samples were collected for ALT,AST,GGT,AKP,TBiL and DBiL test.Bile duct tissues were collected to detect the histolosical changes,MPO activit,and the expression of TNF-α mRNA.Results The serum levels of GGT in group P was significantly lower than that in group N at the 6th hour and 24th hour after reperfusion(P<0.05).And the serum levels of AKP,TBiL and DBiL and the morphological scores of bile duct in group P were significantly lower than that in group N at the 24th hour after reperfusion(P<0.05).The activity of MPO in group N was significantly higher than that in group P at the 6th hour after reperfusion(P<0.05).Compared with group N,the expression of TNF-αmBNA was reduced significantly in group P at the 2nd hour and 6th hour after reperfusion(P<0.05).Conclusion Simultaneaus hepatic artery and portal revascularization can alleviate I/R induced bile duct injury of rat liver grafts,and the protective mechanism may be asseciated with inhibition the expression of TNF-α and decrease of neutrophil infiltration.
Keywords:Hemodynamic processod  Liver transplantation  Tumor necrosis factor-alpha
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号