胃癌组织碱性成纤维细胞生长因子表达及对血管新生和肿瘤进展的影响

目的　探讨碱性成纤维细胞生长因子 (bFGF)在胃癌组织中表达及其对血管新生和肿瘤生物学行为的影响。方法　应用免疫组化SP法检测 74例胃癌 ,17例癌旁组织bFGF表达及间质微血管密度 (MVD)。结果　胃癌组织中肿瘤细胞、间质新生血管高度表达bFGF。癌组织bFGF表达(77.0 3% )明显高于癌旁组织 (2 9.4 1% ,P <0 .0 1)。癌旁胃黏膜及伴有肠上皮化生的胃黏膜表达bFGF较弱。bFGF高表达组的平均MVD值 (79.3± 11.2 )明显高于bFGF低表达组 (71.2± 11.9,P <0 .0 5 )。此外bFGF表达程度与胃癌淋巴结转移和癌浸润深度密切相关。结论　bFGF可促进肿瘤间质微血管生成 ,加速肿瘤浸润和转移。

Expression of bFGF and its effect on tumor interstitial angiogenesis and progression in gastric carcinoma

Objective The purpose of this study was to investigate the expression of basic fibroblast growth factor (bFGF) and its effect on tumor interstitial angiogenesis and the biological behavior of gastric carcinoma. Methods CD34 was used to explore the microvessel density (MVD) as the marker of endothelial cell. bFGF was qualitatively and quantitatively detected with immunohistochemistry on 74 cases of gastric carcinoma and 17 cases of adjacent normal gastric tissue. Results Measurement of CD34 expression could be used to describe endothelial cell proliferation and vascularity. Strong expression of bFGF was localized in the cytoplasm of tumor cell and interstitial new microvessel endothelial cells. Expression of bFGF in tumor tissue was stronger than in corresponding non tumor normal gastric tissue ( P <0.01). The latter and gastric mucosa with intestinal metaplasia weakly expressed bFGF. MVD was significantly higher in the bFGF strong expression group than that in the bFGF weak expression group( P <0.05). In addition, there was a close relationship between the expression degree of bFGF and metastasis of the lymph node and the depth of tumor invasion. Conclusions bFGF might contribute to the angiogenesis and the tumor invasion as well as metastasis in gastric carcinoma on the way of autocrine/paracrine.