单侧输尿管梗阻大鼠肾间质纤维化的发生机制

目的 探讨单侧输尿管梗阻后肾间质纤维化的形成机制.方法 采用单侧输尿管梗阻(uniliteral ureteral obstruction UUO)致肾间质纤维化大鼠模型,将54只大鼠随机分假手术组、梗阻组.术后7天、14天、21天观察肾组织病理改变,并应用免疫组织化学方法检测肾间质核转录因子-κB(Nuclear factor-κB NF-κB)、纤溶酶原激活物抑制剂-1(plasminogen activator inhibitor-1 PAI-1)的表达.结果 随着梗阻时间延长,梗阻组肾间质PAI-1和NF-κB的表达增加,假手术组无明显变化,两组相比有显著差异(p＜0.01).结论 单侧输尿管梗阻肾间质NF-κB、PAI-1的过度表达,是形成间质纤维化的重要原因之一.

The mechanism of renal fibrosis in rats after uniliteral ureteral obstruction

Objectives To investigate the mechanism of renal interstitial fibrosis after unilateral ureteral obstruction. Methods Fifty-four rats were randomly assigned to shame operation group, operation group after unilateral ureteral obstruction. Renal tissues were examined by HE and Masson stain. Immunhistochemistry was performed for Nuclear factor-κB(NF-κB), plasminogen activator inhibitor-1 (PAI-1) in the renal interstitum.Results The fibrosis index of the UUO group was significantly increased (p ＜ 0.05). NF-κB and PAI-1 protein were highly expressed in the UUO group (p ＜ 0. 01). Conclusions The over-expression of PAI-1 and NF-κB maybe one of the important reasons of development of renal fibrosis.