| HPLC法同时测定紫果西番莲叶提取物异荭草素与荭草素的含量 |
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| 引用本文： | 邹江冰,陈龙浩,蒋琳兰.HPLC法同时测定紫果西番莲叶提取物异荭草素与荭草素的含量[J].解放军药学学报,2009,25(5):443-445. |
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| 作者姓名： | 邹江冰 陈龙浩 蒋琳兰 |
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| 作者单位： | 1. 华南理工大学生物科学与工程学院,广东,广州,510640;广州军区广州总医院,药剂科,广东,广州,510010
2. 广州军区广州总医院,药剂科,广东,广州,510010 |
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| 基金项目： | 广州市科委基础性研究计划项目 |
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| 摘 要： | 目的建立紫果西番莲叶提取物中异荭草素和荭草素的含量测定方法。方法采用HPLC法。Inertsil C18色谱柱（4.6mm×250mm,5μm）;流动相：水-四氢呋喃-异丙醇-乙腈（87∶8∶2∶3）;柱温：30℃,流速：1mL/min;检测波长：360nm。结果异荭草素、荭草素回归方程：Y=2.901×10^4X-188.1,r=0.999 8;Y=1.824×10^4X-228.83,r=0.999 5;线性范围分别为0.012～0.602、0.009～0.451mg.mL^-1。平均回收率分别为100.09%、98.66%,RSD分别为2.10%、1.81%。结论HPLC法测定紫果西番莲叶提取物中异荭草素和荭草素的含量,专属性强、精密度好、操作简单、准确性高。
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| 关 键 词： | 紫果西番莲叶 异荭草素 荭草素 含量测定 HPLC |
HPLC Determination of Isorientin and Orientin in Passiflora Incamata Extract Simultaneously |
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| ZOU Jiang-Bing,CHEN Long-Hao,JIANG Lin-Lan.HPLC Determination of Isorientin and Orientin in Passiflora Incamata Extract Simultaneously[J].Pharmaceutical Journal of Chinese People's Liberation Army,2009,25(5):443-445. |
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| Authors: | ZOU Jiang-Bing CHEN Long-Hao JIANG Lin-Lan |
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| Institution: | 1.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, Guangdong China ;2.Dept of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010,Guangdong China) |
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| Abstract: | Aim To establish a method for detecting isorientin and orientin by HPLC. Methods The chromatographic conditions include the Intersil C18 column and a mobile phase consisting of a trinal aquatic solution , i. e. H2O-tetrahydrofuran-isopropanol-acetonitrile （87： 8： 2：3 ）. The flow rate was 1.0mL/min. The detection wave- length was 360nm. The temperature was 30℃. Results The regression equation for isorientin and orientin was：Y = 2.901 × 10^4X - 188.1, （ r = 0.999 8） ; Y = 1. 824 × 10^4X - 228.83 （ r = 0. 999 5 ） respectively ; the linear range was 0.012 -0. 602mg.mL^-1 ,0.009 - 0. 451mg.mL^-1 respectively; the average recovery （ RSD ） was 100.09% （2.10） ,98.66% （ 1.81 ） respectively. Conclusions The method is rapid ,accurate and reliable. |
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| Keywords: | HPLC |
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