| Slug基因表达抑制对体外胰腺癌细胞生物行为的影响 |
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| 引用本文： | 陈彦福,宋彩霞,王全,欧树安,严明总. Slug基因表达抑制对体外胰腺癌细胞生物行为的影响[J]. 中国实验诊断学, 2010, 14(4): 483-486 |
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| 作者姓名： | 陈彦福 宋彩霞 王全 欧树安 严明总 |
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| 作者单位： | 1. 山东胶南市人民医院,普外科,山东,青岛,266400
2. 海南医学院附属医院,普外科,海南,海口,517010 |
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| 基金项目： | 海南省自然科学研究基金项目 |
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| 摘 要： | 目的探讨siRNA干扰Slug基因表达对胰腺癌转移、血管生成和生长的影响。方法用脂质体转染法将表达siRNA-Slug的真核表达载体pGenesil-1-Slug-siRNA(pSlug-siRNA)和空载体pGenesil-1-Neg-siRNA(pNeg-siRNA)导入AS-PC-1细胞,G418筛选阳性细胞,获得稳定转染的阳性克隆。采用RT-PCR技术检测不同处理组细胞中Slug mRNA表达水平,Western-blot测定Slug蛋白表达水平。观察Slug沉默后及对体外胰腺癌细胞侵袭作用的影响;鸡胚绒毛尿囊膜(CAM)试验及CAM移植瘤实验检测Slug沉默对血管生成的影响,MTT比色法检测细胞存活率。结果pSlug-siRNA组细胞内Slug表达被有效沉默。重组细胞基底膜(Matrigel)侵袭实验显示,pSlug-siRNA组、对照组、pNeg-siRNA组穿透基底膜细胞数分别为(38±6.56)、(164±15.39)、(159.67±8.96)个/高倍镜(×200),差异分别有统计学意义(P0.05)。体外实验表明,AsPC-1细胞内Slug表达沉默后,血管密度明显降低,血管生成被抑制,细胞存活率明显降低。结论RNA干扰Slug基因抑制胰腺癌细胞生长、侵袭转移和血管生成,Slug可能成为胰腺癌基因治疗的新靶点。
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| 关 键 词： | 胰腺癌 Slug 干扰 NO瘤转移 血管生成 |
Inhibitory effects of anti-Slug on invasion,migration and angiogenesis in pancreatic carcinoma cells |
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| CHEN Yan-fu,SONG Cai-xia,WANG Quan,et al.. Inhibitory effects of anti-Slug on invasion,migration and angiogenesis in pancreatic carcinoma cells[J]. Chinese Journal of Laboratory Diagnosis, 2010, 14(4): 483-486 |
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| Authors: | CHEN Yan-fu SONG Cai-xia WANG Quan et al. |
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| Affiliation: | CHEN Yan-fu1,SONG Cai-xia1,WANG Quan2,et al.(1.Department of General Surgery,the Jiaonan Manicipal Hospital,Qingdao 266400,China,2.Department of General Surgery,the Affiliated Hospital of Hainan Medical College,Haikou 517010,China) |
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| Abstract: | Objective This study was to inhibit Slug expression in pancreatic cancer cells with small interfering RNA(siRNA),and explore its effect on cell metastasis and angiogenesis.Methods Plasmaids pSlug-siRNA and pNeg-siRNA(negative control)Were constructed and transfected into AsPC-1 cells by Lipofectamine 2000 methods,stable transfected clony was chosen through G418.The expression of Slug was detected by RT-PCR and Western blotting.MTT assay was used to observe the inhibitory actions of pSlug-siRNA on AsPC-1 cel... |
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| Keywords: | Slug |
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