| 威灵仙多糖对人肝癌Bel7402细胞凋亡的影响 |
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| 引用本文： | 张杰,杨旭东,杨骄霞.威灵仙多糖对人肝癌Bel7402细胞凋亡的影响[J].牡丹江医学院学报,2011,32(1):9-11. |
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| 作者姓名： | 张杰 杨旭东 杨骄霞 |
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| 作者单位： | 1. 牡丹江医学院,黑龙江,牡丹江,157011
2. 牡丹江医学院附属红旗医院,黑龙江,牡丹江,157011 |
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| 基金项目： | 黑龙江省卫生厅科研课题(2009-413) |
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| 摘 要： | 目的：探讨威灵仙多糖对人肝癌Bel7402细胞凋亡的影响及其分子机制。方法：不同浓度威灵仙多糖作用于Bel7402细胞48h后,荧光显微镜观察人Bel7402细胞凋亡情况,RT-PCR法检测Bcl-2、Fas基因表达量的变化。结果：威灵仙多糖能诱导Bel7402细胞凋亡,40mg/L时凋亡率为17.63%,实验组与对照组比较有显著性差异;并且上调Fas和降低Bcl-2表达。结论：威灵仙多糖能促进Bel7402细胞凋亡,其机制可能与激活Fas、抑制Bcl-2的基因表达有关。
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| 关 键 词： | 威灵仙多糖 肝癌 Fas Bcl-2 |
THE EFFECT OF RADIX CLEMATIDIS POLYSACCHARIDE ON THE APOPTOSIS OF HUMAN HEPATOCELLULAR CARCIOMA BEL7402 CELLS |
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| Zhang jie etal.THE EFFECT OF RADIX CLEMATIDIS POLYSACCHARIDE ON THE APOPTOSIS OF HUMAN HEPATOCELLULAR CARCIOMA BEL7402 CELLS[J].Journal of Mudanjiang Medical College,2011,32(1):9-11. |
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| Authors: | Zhang jie etal |
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| Institution: | Zhang jie etal(Mudanjiang Medical College,Mudanjiang 157011,china |
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| Abstract: | Objective:To investigate the influence of Radix Clematidis polysaccharide(RCP)on apoptosis of bel7402 cells and reveal the mechanismMethods: Bel7402 cells were treated with various of RCP,We observed cell apoptosis of bel7402 cells by fluorescence microscope.We detected the levels of Bcl-2 and Fas gene expression in all groups by RT-PCR.Results: RCP has obviously apoptosis-inducing effects on bel7402 cells.And apoptotic rate was 17.84% when the density of RCP was 40mg/L.It was found that there was a signifi... |
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| Keywords: | Radix Clematidis polysaccharide Hepatocellular Carcinoma Fas Bcl-2 |
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