牛磺酸对四氯化碳染毒大鼠钙稳态失调的干预规律

本研究中除设立正常对照组，四氯化碳（ＣＣｌ４）对照组外，尚设立２４０ｍｍｏｌ／Ｌ牛磺酸预投组（只在染毒前给予）和２４０ｍｍｏｌ／Ｌ牛磺酸同投组（只在染毒同时给予）来对照考察８０ｍｍｏｌ／Ｌ和２４０ｍｍｏｌ／Ｌ牛磺酸双投（染毒前及染毒过程中均给予）对５００ｍｇ／ＫｇＣＣｌ４经口染毒，２次／周，持续５周实验动物的干预作用。结果如下：１．２４０ｍｍｏｌ／Ｌ牛磺酸双投对Ｃａ２＋－Ｍｇ２＋－ＡＴＰａｓｅ活性有部分保护作用，使其达到对照组的７４．４８％；对Ｃａｍ活性亦有明显的保护作用，并使线粒体钙含量显著降低；８０ｍｍｏｌ／Ｌ双投，２４０ｍｍｏｌ／Ｌ预投和２４０ｍｍｏｌ／Ｌ同投均未有明确的干预作用。２．８０ｍｍｏｌ／Ｌ和２４０ｍｍｏｌ／Ｌ牛磺酸双投作用存在明显差异。２４０ｍｍｏｌ／Ｌ双投明显地防止了Ｃａ２＋－Ｍｇ２＋－ＡＴＰａｓｅ和Ｃａｍ活性的抑制，对线粒体钙含量亦有显著的降低作用，而８０ｍｍｏｌ／Ｌ牛磺酸未见此效果。３．２４０ｍｍｏｌ／Ｌ牛磺酸双投和保护作用明显，优于２４０ｍｍｏｌ／Ｌ同投；２４０ｍｍｏｌ／Ｌ牛磺酸预投的效果不明显。故研究提示应重视牛磺酸干预作用的时效性和量效性。

Protective effects of taurineon disturbance of livercalcium homeostasis induced by carbon tetrachlorid

Male SD rats were treated with 80,240mmol/L taurine in drinking water for 7 days prior to and simultaneously with subacute intoxicantion,CCl 4 dosing 500mg/kg was given two times/wk over 5 weeks.Groups were designed as normal control,CCl 4 treament,240mmol/L taurine pretreatment group(TPG,taurine treatment only prior to CCl 4 treatment) and 240mmol/L taurine simultaneous treatment group(TSG,taurine treatment simultanously with CCl 4 treatment) in contrast to 80,240mmol/L taurine double treatment groups(TDG).The result was shown as follows:(1) In 240mmol/L TDG there were partial protective effect on the activity of Ca 2 Mg 2 ATPase which was increased to 74 48% of that of control and complete protective effect on the activity of Cam,mitochondria calcium content were also decreased significantly; In 80mmol/L TDG,240mmol/L TPG and TSG,taurine all showed no antagonism against the disturbance of liver calcium homeostasis.(2) There were different significantly protective effects between 80 and 240mmol/L TDG.240mmol/L TDG could markedly prevent the activities of Ca 2 Mg 2 ATPase and Cam from inhibition and could increase of mitochomdria calcium content.However,80mmol/L TDG had no effect on calcium homeostasis.(3) The effects of 240mmol/L TDG was superior to that of 240mmol/L TSG and the effect of TPG werent't definite.Therefore,it suggest that it is very important to emphasis the dose effect and time effect relation of taurine.