粉防己碱对缺血顿抑大鼠心肌功能和ATP酶活力的影响

目的：观察粉防己碱（ｔｅｔｒａｎｄｒｉｎｅ，Ｔｅｔ）对缺血顿抑心肌的保护作用并分析与心肌ＡＴＰ酶活力的关系。方法：离体大鼠工作心脏，全心缺血３０ｍｉｎ再灌４０ｍｉｎ，无机磷显色法分析ＡＴＰ酶活力。结果：顿抑心肌收缩舒张功能均明显降低，再灌期末ＬＶＳＰ×ＨＲ仅恢复５２％±８％，ＬＶＥＤＰ抬高２９８％±６４％，ＣＯ仅恢复４０％±８％，心肌细胞膜和线粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活力均下降，Ｔｅｔ（３０ｍｇ·ｋｇ－１·ｄ－１，ｉｐ，３ｄ，１０μｍｏｌ·Ｌ－１灌流缺血全程）可使ＬＶＳＰ×ＨＲ恢复８５％±１２％，ＬＶＥＤＰ仅抬高１６６％±４４％，ＣＯ恢复７５％±１１％，心肌细胞膜及线粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活力增高。结论：Ｔｅｔ对心肌缺血后顿抑损伤有一定保护作用，这一作用与维护顿抑期心肌细胞ＡＴＰ酶活力有关。

Effects of tetrandrine on cardiac function and ATPase activity of postischemic myocardial stunned rat hearts

AIM: To study the protective effect of tetrandrine (Tet) on postischemic myocardial stunning and whether it is responsible for ATPase activity. METHODS: Isolated working rat hearts, global ischemia for 30 min followed by reperfusing for 40 min. RESULTS: Both systolic and diastolic function of stunned myocardium were significantly decreased. The percent recovery of LVSP×HR and CO was 52%±8% and 40%±8%, respectively; LVEDP and T were elevated; while both Na+,K+ ATPase activity and Ca2+,Mg2+ ATPase activity of myocardial membrane and mitochondria were depressed. Tetrandrine (30 mg·kg-1·d-1, ip, 3 d, and 10 μmol·L-1 for isolated heart perfusion) was able to enhance the recovery of LVSP×HR and CO to 89%±12% and 80%±14%, respectively, and reduce LVEDP from 298%±64% to 165%±55%, with an improvement in myocardial membrane and mitochondria Na+,K+ ATPase and Ca2+,Mg2+ ATPase activity. CONCLUTION: This study suggested that tetrandrine can protect cardiac function from ischemia reperfusion stunning injury, which may be related to preserving ATPase activity in ischemic myocardium.