实时荧光定量PCR检测乳腺癌5种多药耐药基因的表达强度

目的 :检测 5种多药耐药基因在乳腺癌细胞株 MCF- 7和 MCF- 7/ADR里的表达强度变化 ,为乳腺癌多药耐药逆转研究提供新的思路。方法 :通过实时荧光定量 PCR技术分别检测乳腺癌敏感细胞株 MCF- 7和耐药细胞株 MCF- 7/ADR里的 mdr1、MRP、L RP、GST- π、TOPO α基因表达强度。结果 :MCF- 7/ADR里的 mdr1、MRP、LRP、GST- π基因表达强度分别比它们在 MCF- 7里的表达强度相对增加了 1 2 5 .39、5 5 .5 3、1 .2 4、3.38倍 ,TOPO α则降低了 0 .43倍 ,其中 mdr1、MRP表达强度的相对增加倍数均显著高于 L RP、GST- π、TOPO α的增加 (或降低 )倍数 ( P<0 .0 0 1 )。结论 :除了 mdr1之外 ,MRP也可能在乳腺癌的多药耐药产生机制里起重要作用。因此 MRP基因可以成为乳腺癌耐药逆转研究的新作用靶点

The detection of five multidrug resistant genes expression level by real-time fluorogenic quantitative RT-PCR

Objective: To detect expression level changes of five multidrug resistant genes in breast cancer cell lines MCF-7 and MCF-7/ADR by real-time FQ- RT-PCR.Methods: The expression levels of mdr1,MRP,LRP,GST-π and TOPOⅡα were measured in breast cancer sensitive cell line MCF-7 and multidrug resistant cell line MCF-7/ADR by FQ-RT-PCR. Results: The expression levels of mdr1,MRP,LRP,GST-π in MCF-7/ADR increased 125.39,55.53,1.24,3.38 times than them in MCF-7,TOPOⅡα decreased 0.43 times. The relative increasing times of mdr1,MRP expression levels were higher than LRP,GST-π,TOPOⅡα(P<0.001). Conclusions : MRP maybe also plays an important role in breast cancer MDR mechanism besides mdr1. MRP can be a new target gene for reversing breast cancer MDR.