|
|||||
|
|
| 低频超声对大鼠主动脉平滑肌细胞凋亡的作用 | |
| 引用本文： | 张波,程茜,陆英,怡兰,陈明. 低频超声对大鼠主动脉平滑肌细胞凋亡的作用[J]. 上海医学影像, 2010, 19(1): 63-65 |
| 作者姓名： | 张波 程茜 陆英 怡兰 陈明 |
| 作者单位： | 1. 同济大学附属东方医院超声科,200120 2. 同济大学声学研究所 3. 中心实验室 4. 内蒙古医学院 |
| 基金项目： | 上海市卫生局青年科研基金项目资助，国家自然科学基金青年科学基金项目，上海市科委浦江人才计划 |
| 摘 要： | 目的观察低频超声波对体外培养的大鼠主动脉平滑肌细胞（VSMCs）凋亡的影响。方法42.6kHz超声辐照大鼠主动脉平滑肌细胞，台盼蓝染色分析辐照后即刻细胞存活率，流式细胞仪观察24小时后细胞凋亡。结果低频超声辐照可以诱导培养的VSMCs凋亡，该作用在50秒内随辐照时间的增加有增加的趋势。结论低频超声在无明显杀伤细胞的情况下可在短时间内诱导培养的VSMCs凋亡，提示低频超声是一种潜在的治疗PCI术后再狭窄的手段。 |
| 关 键 词： | 血管平滑肌细胞 低频超声 凋亡 |
Affection of low frequency ultrasound on the apoptosis of vascular smooth muscle cells from rat aorta |
|
| ZHANG Bo,CHENG Qian,LU Ying,YI Lan,CHEN Ming. Affection of low frequency ultrasound on the apoptosis of vascular smooth muscle cells from rat aorta[J]. Shanghai Medical Imaging, 2010, 19(1): 63-65 | |
| Authors: | ZHANG Bo CHENG Qian LU Ying YI Lan CHEN Ming |
| Affiliation: | ZHANG Bo,CHENG Qian.LU Ying.YI Lan,CHEN Ming (Shanghai East Hospital affiliated to Tongji University,shanghai 200120,China) |
| Abstract: | Objective To observe the effects of low frequency ultrasound on the apoptosis of in vitro cultured vascular smooth muscle cells(VSMCs) from rat aorta.Methods 42.6kHz low frequency ultrasound was applied onto the rat VSMCs.Immediate survival was evaluated by Trypan Blue staining,and the apoptosis after 24 hours was evaluated by flow cytometry.Results Low frequency ultrasound induced apoptosis of cultured VSMCs,which was intensified along with the duration of radiation within 50 seconds. Conclusion Low freque... |
| Keywords: | Vascular smooth muscle cells Low frequency ultrasound Apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |