彩超引导下羊膜腔穿刺术及脐带穿刺术在产前诊断中的应用

目的 探讨彩超引导下羊膜腔穿刺术（AC）及脐带穿刺术（FBS）在产前诊断中的应用价值.方法 对1 008例具有产前诊断指征的孕妇按孕周大小分为两组,孕17～21周943例为AC组,孕22～36周65例为FBS组,分别抽取羊水标本和脐带血标本进行细胞培养,行染色体核型及珠蛋白基因检查.结果 AC组一针成功率100%,未发生孕妇及胎儿并发症,标本母血污染率0.1%;FBS组一针成功率61.5%（40/65）;发现胎儿染色体异常36例,重型地中海贫血16例.结论 彩超引导下羊膜腔穿刺术和脐带穿刺术安全可靠,有较高的成功率,可用于产前诊断.

Application of amniocentesis and fetal blood sampling guided by color Doppler ultrasound in prenatal diagnosis

Objective To investigate the clinical application value of amniocentesis （AC） and fetal blood sampling（FBS） guided by color Doppler ultrasound in prenatal diagnosis. Methods A total of 1 008 pregnant women with prenatal diagnosis indications were divided into two groups according to the pregnant weeks：AC group including 943 cases of pregnancy between 17 and 21 weeks, FBS group including 65 cases of pregnancy between 22 and 36 weeks. The amniotic fluid and fetal blood samples were adopted by color Doppler ultrasound-guided AC or FBS for genetic analysis and karyotyping. Results In AC and FBS groups,the successful rate of first puncture was 100% and 61.5%, respectively. In AC group,the contamination of the maternal blood was 0. 1%, 36 cases of abnormal chromosome and 16 cases of thalassemia major were found. Conclusion Both AC and FBS guided by color Doppler ultrasound are safe and reliable, and they have a high successful rate. They are significant in screening for prenatal diagnosis.