Hepatocyte proliferation in rats after ventromedial hypothalamic lesions: Immunoreactivity patterns of proliferating cell nuclear antigen (PCNA) |
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Authors: | Takayoshi Kiba Katsuaki Tanaka Kazushi Numata Satoru Saito Hisahiko Sekihara |
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Institution: | (1) Third Department of Internal Medicine, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan, JP |
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Abstract: | Ventromedial hypothalamic (VMH) lesions result in increased DNA content in the rat liver. However, little information is
available concerning the proliferative activity and lobular distribution of different cell populations in VMH-lesioned rat
liver. The aim of the present study was to quantitatiely assess these parameters in VMH-lesioned rat liver by measuring proliferating
cell nuclear antigen (PCNA) reactivity. We investigated to determine whether this mitotic response involves acinar zones 1,
2, or 3. Changes in immunohistochemical labeling indices in rat liver were measured with an antibody against PCNA until 7
days after VMH lesioning. The effects of hepatic vagotomy on proliferation were also examined. Proliferation of hepatocytes
in acinar zones 1–3 began to increase on day 1, and reached a maximum at 3 days. The area of most intense proliferation progressively
shifted from acinar zone 1 to zone 3 in several days. The proliferation was completely inhibited by hepatic vagotomy, indicating
that VMH lesions induce cell proliferation in rat liver via hepatic vagus nerve activity. This study implicates the information
available on neural factors which initiate hepatocyte proliferation.
(Received Sept. 4, 1997; accepted Dec. 19, 1997) |
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Keywords: | : ventromedial hypothalamus hepatocyte proliferation immunohistochemistry |
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