| ^99Tc—MDP对类风湿性关节炎患者外周血单核细胞产生细胞因子的影响 |
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| 引用本文： | 吴永刚,马琦琳,等.^99Tc—MDP对类风湿性关节炎患者外周血单核细胞产生细胞因子的影响[J].湖南医科大学学报,2002,27(2):173-175. |
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| 作者姓名： | 吴永刚 马琦琳 |
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| 作者单位： | [1]中南大学湘雅二医院核医学科，长沙410011 [2]中南大学湘雅医院心内科，长沙410008 |
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| 摘 要： | 目的：探讨“云克”（^99Tc-MDP)治疗类风湿性关节炎（RA）的免疫机制。方法：采用ELISA双抗体夹心法，观察“云克”体外对RA患者外周血单核细胞（PBMC）产生白介素1（IL－1）和可溶性白介素2受体（sIL-2R)的影响。结果：“云克”有抑制RA患者IL－1的分泌及细菌脂多糖（LPS）的促分泌作用，并对可溶性白介素2受体的自发分泌及植物血素（PHA）诱导分泌均有抑制作用。结论：“云克”对RA的治疗机制可能与其降低IL－1和sIL-2R的作用有关。
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| 关 键 词： | ^99Tc－MDP 类风湿性关节炎 外周血 单核细胞 细胞因子 影响 白细胞介素1 细胞介素－2 受体 |
Effect of 99Tc-MDP on cytokine production by peripheral blood mononuclear cells of patients with rheumatoid arthritis] |
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| Yong-gang Wu,Qi-lin Ma,Guo-feng Liu.Effect of 99Tc-MDP on cytokine production by peripheral blood mononuclear cells of patients with rheumatoid arthritis][J].Bulletin of Hunan Medical University,2002,27(2):173-175. |
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| Authors: | Yong-gang Wu Qi-lin Ma Guo-feng Liu |
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| Institution: | Department of Nuclear Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. |
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| Abstract: | OBJECTIVE: To study the immune mechanism of 99Tc-MDP in the treatment of patients with rheumatoid arthritis (RA). METHODS: In vitro, 99Tc-MDP was added to the cultures of peripheral blood mononuclear cells (PBMC) derived from healthy subjects and RA patients. The levels of IL-1 and sIL-2R from PBMC affected by 99Tc-MDP were determined with enzyme linked immunosorbert assays (ELISA). RESULTS: The IL-1 production by PBMC of RA, either in the medium alone (spontaneously) or in the presence of lipopolysacchride (LPS) stimulation, was suppressed by 99Tc-MDP; and the sIL-2R by PBMC of RA, either in the medium alone (spontaneously) or in the presence of phytohemagglutinin (PHA) induction, was inhibited by 99Tc-MDP. CONCLUSION: 99Tc-MDP acts on PBMC and a possible immune activity by 99Tc-MDP is related to the suppression of IL-1 and sIL-2R in RA patients. |
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