艾滋病患者HAART治疗前后CD4+T细胞表面某些归巢分子和辅助受体表达变化分析

目的 探讨艾滋病(AIDS)患者高效抗逆转录病毒治疗(HAART)治疗前后CD4+T细胞表面CD49d、CCR9、CD62L和CCR5表达的变化情况.方法 采用流式细胞术检测42例艾滋病患者和18例HIV阴性健康对照的外周血CD4+T细胞表面CIM9d、CD62L、CCR9和CCR5的表达,并对CD4+CCR9+和CD4+CCR5+T细胞进一步进行CD45RO表型分析,采用BD FACSDiva软件分析计算各组细胞表达的百分率.结果 艾滋病患者尚未开始HAART治疗组(pre-HAART组)外周血CD4+细胞计数明显低于HAART治疗组(HAART组)(P<0.01);pre-HAART组外周血单个核细胞(PBMC)中CD3+CD4+,CD4+CCR9+,CD4+ CCR5+T细胞的百分率均明显低于HAART组(P<0.01),pre-HAART组CD4+CD49d+,CD4+CD62L+,CIM+CCR9+CD45RO+,CD4+CCR9+CD45RO-,CD4+CCR5+CD45RO+,CD4+CCR5+CD45RO-T细胞的百分率显著低于HAART组(P<0.001);HAART组PBMC中CD3+CD4+,CD4+CD62L+,CD4+CCB5+T细胞的百分率均显著低于HIV阴性对照(HIV-neg组)(P<0.001);pre-HAART组以上各组细胞群的百分率均显著低于HIV-neg组(P<0.05).结论 AIDS患者外周血CD4+T细胞表面肠道归巢分子CD49d、CCR9,淋巴结归巢分子CD62L,辅助受体CCR5异常改变,但HAART可以逆转以上部分病理变化.肠道归巢分子CD49d、CCR9和淋巴结归巢分子CD62L可作为艾滋病疾病进展和评价机体HAART后免疫重建情况的指标.

The expression of some homing and co-receptor molecules on CD4+ T cells in AIDS patients

Objective To evaluate the expression of CD49d, CCR9, CD62L and CCR5 on CD4+ T cells in AIDS patients before and after HAART. Methods The study was performed in 42 cases of AIDS patients and 18 cases of healthy controls. The expression of CD49d,CCRg,CD62L and CCR5 on CD4+ T cells,and CIM5RO on CD4+ CCCR9+ and CD4+ CCR5+ T cells in AIDS patients and healthy controls were analysed by Flow cytometry.Software BD FACSDiva was used to calculate the percentage of expression. Results The number of peripheral CD4+ T cells in group pre-HAART was decreased compared with group HAART (P < 0.01);the frequency of CD3+ CD4+ , CD4+ CCR9+ , CD4+ CCR5+ T cells in group pre-HAART were decreased compared with group HAART(P < 0.01), the frequency of CD4+ CD49d+ , CD4+ CD62L+ , CD4+ CCR9+ CD45RO+ , CD4+ CCR9+ CD45RO- ,CD4+ CCR5+ CD45RO+ , CD4+ CCR5+ CD45RO-T cells in group pre-HAART were significantly decreased compared with group HAART (P < 0.001) ; the frequency of CD3+ CD4+ , CD4+ CD62L+ , CD4+ CCR5+ T cells in group HAART were siguificandy decreased compared with group HIV-neg (P < 0.05).Conclusions Not only the number hut the function of CD4+ T cells was impaired in AIDS patients: the lower expression frequency of gut homing receptor molecule CD49d and CCRg, lymph node homing molecule CD62L, co-receptor molecule CCRS. HAART can partially reverse this pathological phenomena. CD49d, CCR9 and CD62L may be suggested to indicate the progression of AIDS and immunologic reconstitution after HAART.