丙氨酸依赖型转氨酶高通量快速检测方法的建立

本研究以来源自Vibrio fluvialis JS17的丙氨酸依赖型转氨酶VfTA为研究对象，偶联丙酮酸氧化酶和辣根过氧化物酶，建立了一种基于反应液颜色变化的转氨酶活性测定新方法，反应条件优化后，考察了VfTA活力单位数与反应液400 nm处吸收度的线性关系，并将此法用于商业化转氨酶ATA117活力的研究。结果显示:该法对转氨酶VfTA的活力检测限可达0.45 U/mL，对ATA117的活力检测限可达0.5 U/mL，并且可根据反应液颜色深浅初步判断酶活力情况。

Rapid and sensitive high-throughput determination method for alanine-dependent transaminase

A new method for determining transaminase activity based on the color change of the reaction solution was established, by using alanine-dependent transaminase VfTA from Vibrio fluvialis JS17 as the research object coupled with pyruvate oxidase and horseradish peroxidase. After the optimization of the conditions, the linear relationship between VfTA activity units and the absorbance at 400 nm was investigated. This method was also applied to determine the activity of commercial transaminase ATA117. The results showed that the detection limit of transaminase VfTA activity was up to 0. 45 U/mL and the detection limit of ATA117 activity was up to 0. 5 U/mL. The transaminase activity could be quickly judged according to the color depth of the reaction solution.