| 利用膜蛋白酵母双杂交系统构建人尿道上皮细胞cDNA文库 |
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| 引用本文： | 李玲玲,余敏君,邓湘赢,戴佩,朱翠明,罗丹,廖雅婷,曾焱华. 利用膜蛋白酵母双杂交系统构建人尿道上皮细胞cDNA文库[J]. 南华大学学报(医学版), 2018, 0(5): 478-481 |
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| 作者姓名： | 李玲玲 余敏君 邓湘赢 戴佩 朱翠明 罗丹 廖雅婷 曾焱华 |
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| 作者单位： | 南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南省子靶标新药研究协同创新中心,湖南 衡阳 421001 |
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| 摘 要： | 利用基于分离的泛素介导的膜蛋白酵母双杂交技术,构建人永生尿道上皮细胞(SV-HUC-1)cDNA文库。Trizol法提取人尿道上皮细胞总RNA,并分离纯化其mRNA；以mRNA为模板,合成cDNA第一链、第二链；通过T4 DNA聚合酶将DNA链两端加上5′接头；并将其大于1 kbp的片段与膜蛋白酵母双杂交载体pPR3-N连接,经电转化大肠杆菌感受态细胞,以构建基于分离的泛素介导的膜蛋白酵母双杂交cDNA文库,并检测文库的库容量和随机性。本研究成功构建了人尿道上皮细胞的基于分离的泛素介导的膜蛋白酵母双杂交cDNA文库,为进一步研究泌尿生殖道感染病原体与宿主尿道上皮细胞的相互作用奠定了实验基础。
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| 关 键 词： | 膜蛋白酵母双杂交 人尿道上皮细胞 cDNA文库 |
| 收稿时间： | 2018-05-29 |
| 修稿时间： | 2018-07-15 |
Construction and Identification of SV-HUC-1 cells cDNA Library Based on Split-ubiquitin Membrane Yeast Two-hybrid System |
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| LI Lingling,YU Minjun,DENG Xiangying,DAI Pei,ZHU Cuiming,LUO Dan,LIAO Yating and ZENG Yanhua. Construction and Identification of SV-HUC-1 cells cDNA Library Based on Split-ubiquitin Membrane Yeast Two-hybrid System[J]. Journal of Nanhua University(Medical Edition), 2018, 0(5): 478-481 |
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| Authors: | LI Lingling YU Minjun DENG Xiangying DAI Pei ZHU Cuiming LUO Dan LIAO Yating ZENG Yanhua |
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| Affiliation: | Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China,Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China,Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China,Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China,Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China,Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China,Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China and Institute of Pathogenic Biology, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center of Molecular Target New Drug Study, Hengyang 421001, Hunan, China |
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| Abstract: | |
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| Keywords: | membrane yeast two-hybrid SV-HUC-1 cells cDNA library |
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