| 原代肠系膜动脉平滑肌细胞的分离培养 |
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| 引用本文： | 陈琛,阳芳,洪陈亮,杨丽,王珍,李洁,秦旭平. 原代肠系膜动脉平滑肌细胞的分离培养[J]. 南华大学学报(医学版), 2018, 0(2): 201-205 |
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| 作者姓名： | 陈琛 阳芳 洪陈亮 杨丽 王珍 李洁 秦旭平 |
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| 作者单位： | 南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001,南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001,南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001,南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001,南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001,南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001,南华大学药物药理研究所血管生物学实验室,湖南 衡阳 421001 |
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| 基金项目： | 南华大学归国留学人员启动基金(2010XQD44),南华大学研究生创新项目(0223-0002-00031). |
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| 摘 要： | 取大鼠肠系膜动脉,用I型胶原酶消化,并进行体外培养,倒置相差显微镜观察ASMC 生长状态及特点；免疫组织化学进行细胞鉴定。大鼠肠系膜平滑肌细胞呈典型“峰-谷”样生长,该原代培养细胞对平滑肌特异性肌动蛋白表达阳性。传代细胞生长稳定,可为研究小血管平滑肌细胞特性提供一个较理想的模型。
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| 关 键 词： | 原代培养 肠系膜 动脉平滑肌细胞 大鼠 免疫组化 |
| 收稿时间： | 2017-09-12 |
| 修稿时间： | 2018-03-04 |
Methods of culture of rat mesenteric artery smooth muscle cellsand identification |
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| CHEN Chen,YANG Fang,HONG Chenliang,YANG Li,WANG Zhen,LI Jie and QIN Xuping. Methods of culture of rat mesenteric artery smooth muscle cellsand identification[J]. Journal of Nanhua University(Medical Edition), 2018, 0(2): 201-205 |
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| Authors: | CHEN Chen YANG Fang HONG Chenliang YANG Li WANG Zhen LI Jie QIN Xuping |
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| Affiliation: | Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China,Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China,Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China,Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China,Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China,Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China and Vascular Biology Laboratory, Institute of Pharmacology, niversity of South China,Hengyang 42001, Hunan,China |
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| Abstract: | Mesenteric artery was obtained from rat and digested by type I collagenase, then cultured in vitro. The status and characteristics of mesenteric artery smooth muscle cells (MASMC) were observed ASMC with inverte phase contrast microscope and indentified by immunochemical. MASMC presented typical peak - valley -like growth and express positive for anti-alpha smooth muscle specific actin. SMC steady growth provides an available model for reseaching the specialities of small vessels. |
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| Keywords: | primary culture mesentery artery smooth muscle cell rat immunocytochemistry |
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