| 富H2水对电离辐射所致认知功能损伤的保护作用 |
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| 引用本文： | 刘梦雅,宋建波,高洁,袁慧,王永丽,王新钢,岳娟,秦秀军,李建国,闻建华.富H2水对电离辐射所致认知功能损伤的保护作用[J].中华放射医学与防护杂志,2018,38(6):414-418,465. |
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| 作者姓名： | 刘梦雅 宋建波 高洁 袁慧 王永丽 王新钢 岳娟 秦秀军 李建国 闻建华 |
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| 作者单位： | 030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030032 太原, 山西大医院放疗科,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室,030006 太原, 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室 |
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| 摘 要： | 目的 探讨富H2水对电离辐射所致大鼠认知功能损伤的保护作用。方法 将20只SD大鼠利用随机数表法分为对照组（C）、富H2水组（HRW）、单纯照射组（IR）和富H2水干预组（HRW+IR）4个组，每组5只。通过Morris水迷宫对各组大鼠空间记忆能力进行检测；对超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）等氧化应激指标的变化进行检测；通过实时荧光定量PCR反应对凋亡相关基因表达情况进行研究。结果 大鼠逃避潜伏期4组间差异有统计学意义（F=6.003，P<0.05），4组间的平台象限游泳距离差异具有统计学意义（F=3.850，P<0.05）。其中，与IR组相比，HRW+IR组第3、4和5天的逃避潜伏期明显缩短（P<0.05），原平台象限游泳距离显著延长（P<0.05）。4组间的GSH、8-OHdG、MDA差异均具有统计学意义（F=6.450、5.033、4.113，P<0.05），与IR组相比，脑组织中GSH浓度显著升高（P<0.05），而MDA和8-OHdG浓度显著降低（P<0.05）。PCR结果显示，与IR组相比，HRW+IR组caspase-3、caspase-9基因表达水平均显著下降（t=2.956、3.087，P<0.05），bcl-2基因表达水平显著升高（t=-3.640，P<0.05），bax基因表达水平显著降低（t=5.246，P<0.05）。结论 富H2水可能通过中和氧羟自由基，减少电离辐射的氧化损伤作用，从而影响细胞凋亡过程进而对大脑产生保护作用。
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| 关 键 词： | 富H2水 电离辐射 认知功能损伤 辐射防护 |
| 收稿时间： | 2017/12/6 0:00:00 |
The protective effect of hydrogen-rich water on irradiation-induced cognitive dysfunction |
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| Liu Mengy,Song Jianbo,Gao Jie,Yuan Hui,Wang Yongli,Wang Xingang,Yue Juan,Qin Xiujun,Li Jianguo and Wen Jianhua.The protective effect of hydrogen-rich water on irradiation-induced cognitive dysfunction[J].Chinese Journal of Radiological Medicine and Protection,2018,38(6):414-418,465. |
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| Authors: | Liu Mengy Song Jianbo Gao Jie Yuan Hui Wang Yongli Wang Xingang Yue Juan Qin Xiujun Li Jianguo and Wen Jianhua |
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| Institution: | Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Department of Radiotherapy, Shanxi Dayi Hospital, Taiyuan 030032, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China,Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China and Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006, China |
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| Abstract: | Objective To investigate the protective effect of hydrogen-rich water on rat cognitive dysfunction induced by ionizing radiation. Methods A total of 20 SD rats were randomly divided into four groups with the ramdom number table method:control group (C), hydrogen-rich water group (HRW), irradiation group (IR) and hydrogen-rich water intervention group (HRW+IR), with 5 rats in each group. The spatial memory ability of rats was tested by a morris water maze. The expression of apoptosis-related genes was detected by real-time fluorescence quantitative PCR. The changes of glutathione (GSH), 8-hydroxydeoxy guanosine (8-OHdG) and malondialdehyde (MDA) and SOD were also measured. Results The escape latency (F=6.003, P<0.05) and the swimming distances (F=3.850, P<0.05) of rats in four different groups had statistically significant differences. Compared with the IR group, the escape latency of the HRW+IR group was significantly decreased at 3, 4, 5 d after irradiation (P<0.05), and the swimming distance of this group became much longer (P<0.05). The levels of GSH, 8-OHdG and MDA in these four groups had statistically significant differences (F=6.450, 5.033, 4.113, P<0.05). Compared with IR group, the concentration of GSH was increased (P<0.05), but MDA and 8-OHdG decreased (P<0.05) in the brain tissue of HRW+IR group, and the expressions of caspase-3, caspase-9 and bax genes were reduced (t=2.956,3.087, 5.246, P<0.05), while the expression of bcl-2 gene was enhanced (t=-3.640, P<0.05) in the HRW+IR group. Conclusions Hydrogen-rich water attenuates the oxidative damage of ionizing radiation by neutralizing oxyhydrogen free radicals and thus protects brain from radiation damage. |
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| Keywords: | Hydrogen-rich water Ionizing radiation Cognitive dysfunction Radiation protection |
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