冠心病痰瘀互结证小鼠模型的建立与评价研究

目的]应用冠状动脉左前降支结扎联合高脂饲料喂养建立冠心病痰瘀互结证小鼠模型，以模拟心肌缺血复合动脉粥样硬化（AS）病理特征，从而达到模拟小鼠冠心病痰瘀互结证的病理变化。方法]健康C57BL/6J雄性小鼠随机分为空白组和假手术组，载脂蛋白E基因敲除（ApoE^-/-）雄性小鼠随机分为血瘀组、痰浊组、痰瘀互结组。痰浊组和痰瘀互结组给予高脂饲料喂养12周，其余各组给予普通饲料喂养12周。在8周末时，对血瘀组和痰瘀互结组进行冠状动脉左前降支结扎，假手术组行穿线不结扎。结果]观察各组小鼠一般状态，超声心动图，对小鼠血清血脂水平进行检测，并对心脏组织进行苏木精-伊红（HE）染色、对主动脉组织进行油红O染色，进而评价冠心病痰瘀互结证小鼠模型建立。结论]ApoE^-/-小鼠采用冠状动脉左前降支结扎联合高脂饲料喂养12周，明显出现心肌缺血及AS病理特征，提示本实验方法可成功建立冠心病痰瘀互结证小鼠模型。

Establishment and evaluation of phlegm and blood-stasis syndromn on coronary heart disease model in mice

Objective] In this study,a mouse model of coronary heart disease with phlegm and blood-stasis syndrome was established by coronary artery left anterior descending artery ligation combined with high-fat diet to simulate the pathological features of myocardial ischemia combined with atherosclerosis,so as to achieve the simulation of pathological changes in this syndrome.Methods] Healthy SPF C57BL/6J mice were used in the control group and the sham operation group,and ApoE^-/- mice were used in the blood stasis group,phlegm group and phlegm and blood-stasis group. The phlegm group and the phlegm and blood-stasis group were fed with high-fat diet for 12 weeks,and the other groups were fed with normal feed for 12 weeks. At the end of the 8th week,the left anterior descending coronary artery was ligated in the blood stasis group and the phlegm and blood-stasis group. The sham operation group was not ligated.Results] The general state of each group of mice was observed. Echocardiography was used to detect serum lipid levels in mice. HE staining of heart tissue and oil red O staining of aortic tissue were performed to evaluate the phlegm and blood-stasis syndrome model of coronary heart disease.Conclusion] ApoE^-/- mice were treated with left anterior descending coronary artery ligation combined with high-fat diet for 12 weeks featured with the myocardial ischemia and atherosclerosis pathology,suggesting that this method can successfully establish the model of the coronary heart disease with phlegm and blood-stasis syndrome in mice.