肝脏肿瘤标本资源库的建立、维护及管理

目的 探讨建立以肝癌组织样本为主的肝脏外科生物样本库及相关数据信息化管理的方法和标准化操作规程，为临床与基础研究提供高质量的临床标本资源平台。方法 收集肝脏肿瘤手术患者的血液标本（血清、血浆、白细胞）、组织标本(癌、癌旁、远端正常组织)，将处理好的血液标本于-80℃冰箱保存，新鲜组织置液氮保存，定期随机抽取液氮保存的肿瘤组织样本进行RNA、DNA的提取及鉴定。结果 现收集并保存有完整临床信息及随访资料的肝脏肿瘤患者血液标本22103份，组织标本3319例，其中肝脏肿瘤组织标本807例，包括肝脏恶性肿瘤762例，良性肿瘤23例，肝硬化组织22例。随机抽取组织RNA、DNA鉴定均符合标本质量标准，能够满足后续实验要求。结论 初步建立标准化、规范化的肝脏肿瘤标本库，制定了标准化的标本库维护和管理规程，为肝脏肿瘤基础和临床研究提供了宝贵的实验材料。

Establishment,maintenance and management of liver tumor biobank

objective To study the measures and standard operation procedure for establishing the biobank of liver surgical samples and information management of related data about samples, providing a high quality clinical specimen resource platform for clinical and basic research. Methods Blood samples from patients with liver tumor (serum, plasma, leukocyte) and tissue samples (cancer tissues, tissues adjacent to carcinoma and the distal normal tissues) were collected, and then be kept in -80 ℃ refrigerator or in liquid nitrogen for preserva-tion. Samples were randomly selected for the extraction and identification of RNA and DNA. Results A total of 22 103 blood samples with complete clinical information and follow-up data of liver tumor patients were collected and saved, 807 cases of tissue specimens, of which there were 762 cases of malignant liver tumor, 23 cases of benign tumors, 22 cases of liver cirrhosis. Random tissue specimens of RNA and DNA identifications were preserved in high quality standards and could meet the requirements of the following studies. Conclusion A standardized and normalized liver tumor specimen biobank is established and the standard specimen library maintenance and management procedures are formulated, which provides valuable experimental materials for basic and clinical research for liver tumor.