| MK-801对EAE动物模型轴索损伤的保护作用研究 |
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| 引用本文： | 张文洛,戚晓昆,王起,雷霞.MK-801对EAE动物模型轴索损伤的保护作用研究[J].脑与神经疾病杂志,2009,17(5):321-324. |
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| 作者姓名： | 张文洛 戚晓昆 王起 雷霞 |
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| 作者单位： | 海军总医院神经内科,北京,100037 |
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| 基金项目： | 全军医学卫生十五重点课题科研基金资助项目 |
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| 摘 要： | 目的通过建立实验性自身免疫性脑脊髓炎(EAE)动物模型,对EAE的发病机制和轴索损伤进行研究,并进行药物干预,寻找新的治疗方法。方法选用髓鞘蛋白脂蛋白(PLP139-151)多肽作为抗原免疫雌性SJL/J小鼠,诱发EAE小鼠模型,应用MK-801(0.30mg/kg)进行干预。发病后取脑、脊髓组织切片,进行免疫组织化学检查。结果模型组23(23/30)只小鼠发病,发病率为76.7%,平均发病时间为19±2.58天,平均神经功能评分为2.14±0.69分。药物(MK-801)治疗组16(16/30)只小鼠发病,发病率为53.3%,平均发病时间为21±1.25天(P<0.05),平均神经功能评分为1.08±0.42分(P<0.02);病理显示药物干预后病灶减少,病变减轻。结论PLP多肽诱发SJL/J小鼠的EAE动物模型的病理改变主要累及脊髓。谷氨酸的兴奋性毒性参与MS发病和轴索损伤,应用NMDA受体拮抗剂MK-801可以减少神经功能缺失,降低发病率。
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| 关 键 词： | 实验性自身免疫性脑脊髓炎 轴索 髓鞘脱失 地卓西平马来酸盐 |
The study of MK-801 therapied axonal damage of experimental autoimmune encephalomyelitis |
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| ZHANG Wen-luo,QI Xiao-kun,WANG Qi,LEI Xia.The study of MK-801 therapied axonal damage of experimental autoimmune encephalomyelitis[J].Journal of Brain and Nervous Diseases,2009,17(5):321-324. |
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| Authors: | ZHANG Wen-luo QI Xiao-kun WANG Qi LEI Xia |
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| Institution: | ZHANG Wen-luo,QI Xiao-kun,WANG Qi,LEI Xia.Department of Neurology,Navy General Hospital,Beijing 100037,China |
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| Abstract: | Objective Our experiment focused on the mechanism of experimental autoimmune encephalomyelitis(EAE) and axonal injury by establishing animal model of EAE combined with drug intervention,and to find new therapeutic ways in EAE by using of dizocilpine maleate(MK-801).Methods Female SJL/J mice immunized with the p139-151 peptide of myelin proteolipid protein(PLP) developed EAE,received intervention of MK-801.Mice were killed when the first neurological attack and their brains and spinal cords tissue samples we... |
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| Keywords: | Experimental autoimmune encephalomyelitis Axon Demyelinating Dizocilpine maleate |
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